In Situ Quantification of Biological N 2 Production Using Naturally Occurring 15 N 15 N
In Situ Quantification of Biological N 2 Production Using Naturally Occurring 15 N 15 N
复制标题
使用天然存在的 15 N 15 N 进行生物 N 2 生产的原位定量
DOI：
10.1021/acs.est.9b00812
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发表时间：
2019
影响因子：
11.4
通讯作者：
Jetten, Mike S. M.
中科院分区：
文献类型：
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作者：
Yeung, Laurence Y.;Haslun, Joshua A.;Ostrom, Nathaniel E.;Sun, Tao;Young, Edward D.;van Kessel, Maartje A. H. J.;Lücker, Sebastian;Jetten, Mike S. M.
我们描述了一种方法来确定生物N2生产的基础上自然发生的15 N15 N在N2的比例在土壤中。实验室培养实验表明，生物N2生产，无论是通过反硝化或厌氧氨氧化，产生的比例15 N 15 N的N2是在1‰的预测随机分布的15 N和14 N原子。这种相对不变的同位素特征与大气形成对比，大气中15 N15 N的比例比随机分布高出19.1 ± 0.1‰。从凯洛格生物站长期生态研究网站农业土壤中的气体的深度剖面显示生物N2积累，占土壤N2的1.6%。这些土壤剖面的一维反应扩散模型表明，地下N2脉冲导致表面排放率低至0.3 mmol N2 m-2d-1可以检测到与当前的分析精度，解耦N2 O生产。
We describe an approach for determining biological N2production in soils based on the proportions of naturally occurring15N15N in N2. Laboratory incubation experiments reveal that biological N2production, whether by denitrification or anaerobic ammonia oxidation, yields proportions of15N15N in N2that are within 1‰ of that predicted for a random distribution of15N and14N atoms. This relatively invariant isotopic signature contrasts with that of the atmosphere, which has15N15N proportions in excess of the random distribution by 19.1 ± 0.1‰. Depth profiles of gases in agricultural soils from the Kellogg Biological Station Long-Term Ecological Research site show biological N2accumulation that accounts for up to 1.6% of the soil N2. One-dimensional reaction-diffusion modeling of these soil profiles suggests that subsurface N2pulses leading to surface emission rates as low as 0.3 mmol N2m–2d–1can be detected with current analytical precision, decoupled from N2O production.