Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model
Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model
复制标题
DOI：
10.1089/ten.2005.11.469
复制
发表时间：
2005-03-01
影响因子：
--
通讯作者：
Ishikawa, I
中科院分区：
文献类型：
--
作者：
Hasegawa, M;Yamato, M;Ishikawa, I
传统的牙周再生方法仍然不足以实现牙周组织的完全和可靠的临床再生。我们开发了一种利用细胞片工程进行细胞移植的新方法，并将其应用于这一问题。本研究的目的是研究从独特的温度响应型培养皿中回收的人牙周膜(HPDL)细胞膜的特性，并检测这些细胞膜是否能够再生牙周组织。对HPDL细胞片进行组织学和生化检查，并将其移植到裸鼠近侧裂开模型中。HPDL细胞是从培养皿中获得的，作为一个连续的细胞片，含有丰富的细胞外基质，并保留完整的整合素，对胰酶-EDTA处理敏感。在动物研究中，在所有移植了HPDL细胞片的无细胞大鼠中，都发现了牙周韧带样组织，其中包括一层无细胞牙骨质样层和锚定在这一层中的纤维。这种纤维锚定与天然牙周韧带纤维非常相似；在未移植的对照组中没有观察到这种再生。这些结果表明，该技术基于细胞片工程的概念，可以用于牙周组织的再生。
Conventional periodontal regeneration methods remain insufficient to attain complete and reliable clinical regeneration of periodontal tissues. We have developed a new method of cell transplantation using cell sheet engineering and have applied it to this problem. The purpose of this study was to investigate the characteristics of human periodontal ligament (HPDL) cell sheets retrieved from culture on unique temperature-responsive culture dishes, and to examine whether these cell sheets can regenerate periodontal tissues. The HPDL cell sheets were examined histologically and biochemically, and also were transplanted into a mesial dehiscence model in athymic rats. HPDL cells were harvested from culture dishes as a contiguous cell sheet with abundant extracellular matrix and retained intact integrins that are susceptible to trypsin-EDTA treatment. In the animal study, periodontal ligament-like tissues that include an acellular cementum-like layer and fibrils anchoring into this layer were identified in all the athymic rats transplanted with HPDL cell sheets. This fibril anchoring highly resembles native periodontal ligament fibers; such regeneration was not observed in nontransplanted controls. These results suggest that this technique, based on the concept of cell sheet engineering, can be useful for periodontal tissue regeneration.