How do cultured CD8(+) murine T cell clones survive repeated ligation of the TCR?
How do cultured CD8(+) murine T cell clones survive repeated ligation of the TCR?
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培养的 CD8( ) 鼠 T 细胞克隆如何在 TCR 的反复连接下存活？
DOI：
10.1093/intimm/14.1.23
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发表时间：
2002
影响因子：
4.4
通讯作者：
Chen,Jianzhu
中科院分区：
文献类型：
--
作者：
Sugawa,Satoshi;Palliser,Deborah;Eisen,HermanN;Chen,Jianzhu
许多鼠T细胞克隆在培养物中持续生长，尽管它们的TCR被抗原每周连接。为了了解培养的细胞如何避免或最小化抗原诱导的细胞死亡（AICD），我们将几种长期培养的CD 8 +T细胞克隆上的Fas和肿瘤坏死因子（TNF）受体（TNFR）与表达相同TCR的幼稚和活化幼稚细胞上的那些进行比较（2C）。与初始细胞相比，培养的克隆上不存在Fas，并且最初在培养的细胞上以高水平存在的TNFR-II受体响应于TCR连接而迅速下调，并且在2 h时几乎消失，此时仅约10%的克隆细胞被诱导表达TNF-α。培养的克隆响应于用于常规刺激培养物的呈递细胞上的同源肽-MHC的AICD的程度也显著小于暴露于高密度抗CD 3抗体板结合后克隆的大量细胞死亡。
Many murine T cell clones grow continuously in culture despite weekly ligation of their TCR by antigen. To learn how the cultured cells avoid or minimize antigen-induced cell death (AICD), we compared Fas and tumor necrosis factor (TNF) receptors (TNFR) on several long-term cultured CD8+T cell clones with those on naive and activated naive cells expressing the same TCR (2C). In contrast to the naive cells, Fas was absent on the cultured clones and the TNFR-II receptor, present initially at high levels on the cultured cells, was rapidly down-modulated in response to TCR ligation and had virtually disappeared by 2 h, when only ~10% of the cloned cells had been induced to express TNF-α. The extent of AICD of the cultured clones in response to cognate peptide–MHC on the presenting cells used for routine stimulation of the cultures was also considerably less than the massive cell death of the clones following exposure to anti-CD3 antibody plate-bound at high density.