Analysis and comparison of the internal promoter, pE, of the ilvGMEDA operons from Escherichia coli K-12 and Salmonella typhimurium.
Analysis and comparison of the internal promoter, pE, of the ilvGMEDA operons from Escherichia coli K-12 and Salmonella typhimurium.
复制标题
大肠杆菌 K-12 和鼠伤寒沙门氏菌 ilvGMEDA 操纵子的内部启动子 pE 的分析和比较。
DOI：
10.1093/nar/14.6.2779
复制
发表时间：
1986
影响因子：
14.9
通讯作者：
Lawther,RP
中科院分区：
文献类型：
--
作者：
Lopes,JM;Lawther,RP
先前确定，尽管将转座子插入ilvG和ilvE中，ilvGMEDA操纵子的远端部分仍被表达。该观察结果表明在ilvE（pE）和ilvD（pD）上游存在内部启动子。已经在体内和体外分析了负责部分ilvEDA表达的内部启动子pE。结果表明：（1）pE在E. coli K-12和S.鼠伤寒;（2）pE位于ilvM编码序列的远端;（3）pE序列在两种细菌中高度保守;（4）ilvM基因产物的氨基酸序列在两种细菌之间具有93%的同源性;（5）从pE的转录可以在体内和体外证明;（6）pE的效率在两种细菌中基本等同。
It was previously determined that the distal portion of the ilvGMEDA operon was expressed despite the insertion of transposons into ilvG and ilvE. This observation suggested the existence of internal promoters upstream of ilvE (pE) and ilvD (pD). The internal promoter pE, responsible for part of ilvEDA expression, has been analyzed both in vivo and in vitro. Our results indicate that: (1) pE exists in both E. coli K-12 and S. typhimurium; (2) pE is located in the distal end of the ilvM coding sequence; (3) the pE sequence is highly conserved in the two bacteria; (4) the amino acid sequence of the ilvM gene product is 93% homologous between the two bacteria; (5) transcription from pE can be demonstrated both in vivo and in vitro; (6) the efficiency of pE is essentially equivalent in the two bacteria.