Application of liquid chromatography/thermospray mass spectrometry to studies on the formation of glutathione and cysteine conjugates from monomethylcarbamate metabolites of bambuterol.
Application of liquid chromatography/thermospray mass spectrometry to studies on the formation of glutathione and cysteine conjugates from monomethylcarbamate metabolites of bambuterol.
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应用液相色谱/热喷雾质谱研究班布特罗单甲基氨基甲酸酯代谢物形成谷胱甘肽和半胱氨酸缀合物。
DOI：
10.1002/rcm.1290031012
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Baillie,TA
中科院分区：
文献类型：
--
作者：
Rashed,MS;Pearson,PG;Han,DH;Baillie,TA
液相色谱/热喷雾质谱法（LC/TSP-MS）已被用于鉴别和研究S-（N-甲基氨基甲酰基）半胱氨酸和S-（N-甲基氨基甲酰基）谷胱甘肽的形成速率，它们分别是半胱氨酸和谷胱甘肽与支气管扩张剂前药班布特罗的两种单甲基氨基甲酸酯代谢产物的体外反应产物。感兴趣的缀合物提供MH+物质，并产生丰富的结构信息片段离子，其用于开发定量、选择性离子监测测定。可以得出结论，LC/TSP-MS代表了一种快速方便的方法，直接水相分析的类S-（N-烷基氨基甲酰基）共轭半胱氨酸和谷胱甘肽。
Liquid chromatography/thermospray mass spectrometry (LC/TSP‐MS) has been used to identify and study the rates of formation of S‐(N‐methylcarbamoyl) cysteine and S‐(N‐methylcarbamoyl)glutathione as products of thein vitroreaction of cysteine and glutathione, respectively, with two monomethylcarbamate metabolites of the bronchodilator pro‐drug bambuterol. The conjugates of interest afforded MH+species and yielded abundant structurally informative fragment ions which were employed in the development of quantitative, selected‐ion monitoring assays. It is concluded that LC/TSP‐MS represents a rapid and convenient approach to the direct aqueous‐phase analysis of the class of S‐(N‐alkylcarbamoyl) conjugates of cysteine and glutathione.