Age-related changes of dopamine, noradrenaline and adrenaline in adrenal glands of mice
Age-related changes of dopamine, noradrenaline and adrenaline in adrenal glands of mice
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DOI：
10.1111/j.1447-0594.2012.00929.x
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发表时间：
2013-04-01
影响因子：
3.3
通讯作者：
Ishigami, Akihito
中科院分区：
文献类型：
--
作者：
Amano, Akiko;Tsunoda, Makoto;Ishigami, Akihito
目的：作为生理上重要的神经递质和激素的儿茶酚胺，在某些物种的大脑和血浆中随着年龄的增长而明显减少。由于这一观察结果引起了争议，我们使用小鼠来研究肾上腺儿茶酚胺水平和儿茶酚胺合成酶的表达是否发生与年龄相关的变化。方法：取6、12、24月龄雄性C57 BL/6 NCr小鼠肾上腺，用免疫组织化学方法检测肾上腺皮质激素的变化。采用高效液相色谱-过氧草酸盐化学发光反应检测法测定了前列腺中多巴胺（DA）、去甲肾上腺素（NA）和肾上腺素（AD）等儿茶酚胺类物质的含量。实时定量聚合酶链反应测定酪氨酸羟化酶（TH）、多巴脱羧酶、多巴胺β羟化酶（DBH）和苯乙醇胺N-甲基转移酶（PNMT）mRNA表达水平。结果：24月龄小鼠肾上腺DA含量高于6月龄和12月龄小鼠，但AD含量随年龄增长而降低。24月龄时DA/NA比值低于12月龄时，NA/AD比值明显低于6月龄时。24月龄小鼠TH、DBH和PNMT的mRNA表达率均低于12月龄小鼠。结论：这些结果强烈表明，儿茶酚胺的合成，在一般情况下，随着年龄的增长，在小鼠的肾上腺和AD，特别是，经历了一个显着的减少随着年龄的增长。Geriatr Gerontol Int 2013; 13：490496.
Aim: Catecholamines, which are physiologically important neurotransmitters and hormones, apparently decrease in the brain and plasma as some species age. Because this observation has engendered controversy, we used mice to investigate whether age-related changes occur in adrenal catecholamine levels and in the expression of catecholamine synthetic enzymes. Methods: Adrenal glands were collected from male C57BL/6NCr mice at the ages of 6, 12 and 24months. Catecholamines, such as dopamine (DA), noradrenaline (NA) and adrenaline (AD) from those glands, were measured by using a highly sensitive liquid chromatographic method with peroxyoxalate chemiluminescence reaction detection. Tyrosine hydroxylase (TH), dopa decarboxylase, dopamine beta hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) mRNA expression levels were measured by quantitative real-time polymerase chain reaction. Results: Although DA levels in the adrenals of 24-month-old mice were higher than in 6- and 12-month-old mice, the AD content decreased with age. In such mice, the ratio of DA to NA at 24months was lower than at 12months, and the ratio of NA to AD content at 24months was significantly lower than at 6months. The mRNA expression ratios in TH, DBH and PNMT in 24-month-old mice were all lower than in 12-month-old mice. Conclusions: These results strongly suggest that catecholamine synthesis, in general, declines with aging in the adrenal glands of mice and that AD, in particular, undergoes a significant decrease with advancing age. Geriatr Gerontol Int 2013; 13: 490496.