Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis.
Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis.
复制标题
慢性鼻窦炎患者鼻息肉组织中抗菌 PLUNC 蛋白表达减少。
DOI：
10.1111/j.1398-9995.2012.02848.x
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发表时间：
2012-07
期刊：
影响因子：
12.4
通讯作者：
Schleimer RP
中科院分区：
文献类型：
--
作者：
Seshadri S;Lin DC;Rosati M;Carter RG;Norton JE;Suh L;Kato A;Chandra RK;Harris KE;Chu HW;Peters AT;Tan BK;Conley DB;Grammer LC;Kern RC;Schleimer RP
慢性鼻窦炎（CRS）是一种以鼻粘膜和副鼻窦炎症为特征的疾病。这种炎症可能部分是由于上皮屏障和先天免疫反应降低，导致频繁的细菌和真菌定植。本研究的目的是探讨先天性免疫蛋白的腭肺和鼻上皮克隆（PLYP）家庭在CRS患者的表达。鼻组织样本采集自对照受试者和CRS患者（有或无鼻息肉）。通过实时PCR分析了PLITERA家族成员的表达。通过ELISA、免疫印迹和免疫组化分析SPLUNC 1和LPLUNC 2蛋白的表达。与对照受试者或CRS患者的钩突组织相比，鼻息肉（NP）中大多数PL家族成员的mRNA水平显著降低。与来自对照受试者的钩突组织相比，CRS患者的NP中的LPLUNC2和SPLUNC1蛋白减少。免疫组化数据显示，在鼻腔鼻窦组织的粘膜下腺体，SPLUNC1和LPLUNC2的差异表达，分别在浆液和粘液细胞。这些分子表达的减少可能是由于NP中腺体数量的减少，如与腺体标志物乳铁蛋白水平的相关性所揭示的。NP中SPLUNC 1和LPLUNC 2的减少反映了粘膜下腺体数量的显著减少。腺体减少可能导致腺体先天防御分子的产生和释放的局部缺陷。
Chronic rhinosinusitis (CRS) is a disease characterized by inflammation of the nasal mucosa and paranasal sinuses. This inflammation may result in part from decreased epithelial barrier and innate immune responses, leading to frequent bacterial and fungal colonization. The objectives of this study were to investigate the expression of innate immune proteins of the Palate Lung and Nasal epithelium Clone (PLUNC) family in patients with CRS. Nasal tissue samples were collected from control subjects and CRS patients with and without nasal polyps. Expression of the members of the PLUNC family was analyzed by real-time PCR. Expression of SPLUNC1 and LPLUNC2 proteins was analyzed by ELISA, immunoblot and immunohistochemical analysis. Levels of mRNA for most of the members of the PLUNC family were profoundly reduced in nasal polyps (NPs) compared to uncinate tissue from control subjects or CRS patients. LPLUNC2 and SPLUNC1 proteins were decreased in NPs of CRS patients compared to uncinate tissue from control subjects. Immunohistochemical data revealed that within submucosal glands of sinonasal tissues, SPLUNC1 and LPLUNC2 were differentially expressed, in serous and mucous cells, respectively. The decrease in expression of these molecules is probably explained by a decrease in the number of glands in NPs as revealed by correlations with levels of the glandular marker lactoferrin. Decreased SPLUNC1 and LPLUNC2 in NPs reflects a profound decrease in the number of submucosal glands. Decreased glands may lead to a localized defect in the production and release of glandular innate defense molecules.
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影响因子：
3.7
作者：
McGillivary G;Bakaletz LO
通讯作者：
Bakaletz LO
影响因子：
3.9
作者：
Ghafouri, B;Kihlström, E;Lindahl, M
通讯作者：
Lindahl, M
影响因子：
2.6
作者：
Berger, G;Kattan, A;Ophir, D
通讯作者：
Ophir, D
DOI：
10.4049/jimmunol.0801375
发表时间：
2009-06-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kato A;Chustz RT;Ogasawara T;Kulka M;Saito H;Schleimer RP;Matsumoto K
通讯作者：
Matsumoto K
影响因子：
14.2
作者：
Tieu, David D.;Peters, Anju T.;Carter, Roderick T.;Suh, Lydia;Conley, David B.;Chandra, Rakesh;Norton, James;Grammer, Leslie C.;Harris, Kathleen E.;Kato, Atsushi;Kern, Robert C.;Schleimer, Robert P.
通讯作者：
Schleimer, Robert P.