Quantitative and qualitative analysis of Artemisiae Verlotori Folium and Artemisiae Argyi Folium by high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry
Quantitative and qualitative analysis of Artemisiae Verlotori Folium and Artemisiae Argyi Folium by high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry
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DOI：
10.1002/jssc.202300063
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发表时间：
2023-03-10
影响因子：
3.1
通讯作者：
Wu，Menghua
中科院分区：
文献类型：
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作者：
Deng，Jing;Ye，Lifang;Wu，Menghua
自古以来，艾叶就被广泛使用。在岭南地区（中国南方），蒿叶verlotorumLamotte，这是命名为红脚艾（HJA），因为根是红色的（红脚的意思是红色的脚），已被用作当地的替代品。这种植物有着悠久的药用和食用历史，可以追溯到晋代。然而，目前尚无系统可靠的方法来控制毛蕊花的质量。本研究建立了高效液相色谱-二极管阵列检测器-四极杆飞行时间高分辨质谱联用技术，用于鉴别和定量毛蕊花和艾蒿中的8种成分（包括有机酸和黄酮类化合物），并建立了毛蕊花和艾蒿的高效液相色谱指纹图谱。同时，采用正交偏最小二乘判别分析和聚类分析，进一步分析了两个品种化学成分的差异。本研究不仅探讨了紫背天葵和艾叶在8个成分上的异同，而且为紫背天葵的质量评价提供了一种快速、准确、全面的定性和定量分析方法。
Artemisiae Argyi Folium (Aiye in Chinese) has been widely used since ancient times. In the Lingnan region (Southern China), the leaf ofArtemisia verlotorumLamotte, which is named Hongjiaoai (HJA) because the roots are red (Hongjiao means red foot in Chinese), has been used as a local substitute for Artemisiae Argyi Folium. The plant has a long medicinal and edible history that can be traced to the Jin Dynasty. However, there is no systematic and reliable method to control the quality of Artemisiae Verlotori Folium. In this study, a comprehensive method involving high‐performance liquid chromatography coupled with diode array detection and quadrupole‐time‐of‐flight high‐definition mass spectrometry was established to identify and quantify eight constituents (including organic acids and flavonoids) in Artemisiae Verlotori Folium and Artemisiae Argyi Folium as well as high‐performance liquid chromatography fingerprints of the two varieties. Moreover, dissimilarities of chemical compositions among the two varieties were further investigated by orthogonal partial least squares discrimination analysis and cluster analysis. This research not only explored the similarities and differences between Artemisiae Verlotori Folium and Artemisiae Argyi Folium in eight components but also provided a qualitative and quantitative analytical method that quickly, accurately, and comprehensively assesses the quality of Artemisiae Verlotori Folium.