A human placental cDNA clone that encodes nonhistone chromosomal protein HMG-1.
A human placental cDNA clone that encodes nonhistone chromosomal protein HMG-1.
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DOI：
10.1093/nar/17.3.1197
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发表时间：
1989-02
影响因子：
14.9
通讯作者：
Lisa Wen;Jenq-Kuen Huang;Barbara H. Johnson;G. Reeck
中科院分区：
文献类型：
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作者：
Lisa Wen;Jenq-Kuen Huang;Barbara H. Johnson;G. Reeck
从人胎盘λ gt 11 cDNA文库中，我们已经分离出了编码HMG-1的整个215个氨基酸残基序列的cDNA克隆。内部序列相似性分析表明，HMG-1的DNA结合结构域是由一个相当长的和灵活的接头段分开。DNA经BamHI酶切后进行Southern杂交，结果表明不同物种中HMG-1基因（或假基因）的数目差异很大。通过北方印迹分析HMG-1 mRNA表达的特征表明，约1.0、1.4和2.4 kb的三种mRNA在所检测的所有哺乳动物器官和细胞系中表达。其中包括处于不同发育阶段的几个大鼠器官。北方分析还表明HMG-1 mRNA在无脊椎动物和植物物种中的存在。
From a human placental lambda gt11 cDNA library, we have isolated a cDNA clone that encodes the entire 215-residue amino acid sequence of HMG-1. Analysis of an internal sequence similarity suggests that the DNA-binding domains of HMG-1 are separated by a rather long and flexible linker segment. Southern blotting of DNA digested with BamHI indicated a highly variable number of genes (or pseudogenes) for HMG-1 in different species. Characterization of HMG-1 mRNA expression by Northern blotting showed that three mRNA species of approximately 1.0, 1.4 and 2.4 kb were expressed in all mammalian organs and cell lines examined. These included several rat organs at different stages of development. Northern analysis also suggested the occurrence of HMG-1 mRNA in an invertebrate and a plant species.