High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription.
High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription.
复制标题
大肠杆菌染色体的高分辨率绘图揭示了高转录和低转录的位置。
DOI：
10.1016/j.cels.2019.02.004
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发表时间：
2019
期刊：
影响因子：
9.3
通讯作者：
Freddolino,PeterL
中科院分区：
文献类型：
--
作者：
Scholz,ScottA;Diao,Rucheng;Wolfe,MichaelB;Fivenson,ElayneM;Lin,XiaoxiaNina;Freddolino,PeterL
Recent studies on targeted gene integrations in bacteria have demonstrated that chromosomal location can substantially affect a gene's expression level. However, these studies have only provided information on a small number of sites. To measure position effects on transcriptional propensity at high resolution across the genome, we built and analyzed a library of over 144,000 genome-integrated, standardized reporters in a single mixed population ofEscherichia coli. We observed more than 20-fold variations in transcriptional propensity across the genome when the length of the chromosome was binned into broad 4 kbp regions; greater variability was observed over smaller regions. Our data reveal peaks of high transcriptional propensity centered on ribosomal RNA operons and core metabolic genes, while prophages and mobile genetic elements were enriched in less transcribable regions. In total, our work supports the hypothesis thatE. colihas evolved gene-independent mechanisms for regulating expression from specific regions of its genome.
