Immune and proliferative cellular responses to Helicobacter pylori infection in the gastric mucosa of Mexican children
Immune and proliferative cellular responses to Helicobacter pylori infection in the gastric mucosa of Mexican children
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DOI：
10.1111/j.1523-5378.2007.00493.x
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发表时间：
2007-06-01
期刊：
影响因子：
4.4
通讯作者：
Torres, J.
中科院分区：
文献类型：
--
作者：
Munoz, Leopoldo;Camorlinga, M.;Torres, J.
背景：幽门螺杆菌感染主要发生在儿童时期，但很少有关于这一年龄组先天免疫和对这种感染的增殖反应的研究。墨西哥儿童幽门螺杆菌患病率高，但胃癌风险低。本研究的目的是研究墨西哥儿童胃粘膜对这种感染的细胞反应。方法：对44例幽门螺旋杆菌感染儿童（平均年龄12 +/- 3.2岁）和44例未感染儿童（平均年龄10 +/- 3岁）进行胃窦和胃主体活检。通过免疫组织化学研究粘膜细胞反应，使用抗ki67抗体进行增殖研究，使用抗人胰蛋白酶进行肥大细胞研究，使用抗人CD68进行巨噬细胞研究。T淋巴细胞和B淋巴细胞用商用综合系统染色。使用KS300软件对细胞反应强度进行组织学估计。结果：幽门螺杆菌感染患儿的上皮细胞增殖和巨噬细胞、T淋巴细胞、B淋巴细胞的浸润明显高于未感染患儿。在感染儿童中观察到CD4、CD8和CD20淋巴细胞的平衡增加。然而，肥大细胞活化减少，中性粒细胞和单核细胞浸润低。上皮细胞增殖与多形核浸润有关，但与巨噬细胞或淋巴细胞浸润无关。CagA（+）感染儿童的炎症和增殖较高。结论：墨西哥儿童对幽门螺杆菌感染的反应是低炎症反应，T淋巴细胞和B淋巴细胞的平衡增加，以及高再生活性。
Background: Helicobacter pylori infection occurs mostly during childhood, but few studies on this age group have addressed the innate immune and the proliferative response to this infection. Mexico has a high H. pylori prevalence in children, but a low risk of gastric cancer. The aim of this work was to study the cellular responses of the gastric mucosa to this infection in Mexican children. Methods: Antral and corpus gastric biopsies were obtained from 44 H. pylori-infected children (mean age 12 +/- 3.2 years) and 44 uninfected children (mean age 10 +/- 3 years). Mucosal cellular responses were studied by immunohistochemistry, using anti-Ki67 antibodies for proliferation studies, antihuman tryptase for mast cells, and antihuman CD68 for macrophages. T and B lymphocytes were stained with a commercial integrated system. The intensity of cellular responses was estimated histologically using the software KS300. Results: Epithelium proliferation and infiltration of macrophages and T and B lymphocytes were significantly higher in H. pylori-infected than in uninfected children. A balanced increase of CD4, CD8, and CD20 lymphocytes was observed in infected children. However, activated mast cells were decreased, and infiltration of neutrophil and mononuclear cells was low. Epithelial proliferation was associated with polymorphonuclear infiltration but not with infiltration of macrophages or lymphocytes. Inflammation and proliferation was higher in CagA (+)-infected children. Conclusions: Mexican children respond to H. pylori infection with a low inflammatory response, a balanced increase of T and B lymphocytes, and a high regenerative activity.