Visible-Light-Initiated Thiol-Acrylate Photopolymerization of Heparin-Based Hydrogels
Visible-Light-Initiated Thiol-Acrylate Photopolymerization of Heparin-Based Hydrogels
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DOI：
10.1021/bm501543a
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发表时间：
2015-02-01
影响因子：
6.2
通讯作者：
Kornfield, Julia A.
中科院分区：
文献类型：
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作者：
Fu, Amy;Gwon, Kihak;Kornfield, Julia A.
使用曙红Y作为光引发剂，三乙醇胺作为电子供体，以引发硫醇化肝素与丙烯酸酯封端的聚（乙二醇）的反应，配制在可见光下固化的原位肝素基形成水凝胶。给出了用于控制肝素含量（1.6至3.3%w/v）、模量（100-10 000 Pa）和胶凝时间（30-600 s）的制剂和辐照条件。在水凝胶中包封3 T3成纤维细胞在所有检查条件下均产生超过96%的活力。从水凝胶中释放的表皮生长因子的体外表征证实了生长因子仍然具有生物活性。提供生长因子的能力，在可见光下的快速凝胶动力学，以及物理和生化特性的独立控制，使该系统成为再生医学的一个有前途的候选者。特别地，在150秒内实现凝胶化的照射条件与视网膜的严格的光暴露极限相容，这为与其他组织一起使用提供了宽的安全裕度。
An in situ heparin-based forming hydrogel that cures under visible-light is formulated using eosin Y as a photoinitiator with triethanolamine as an electron donor to initiate reaction of thiolated-heparin with acrylate-ended poly(ethylene glycol). Formulations and irradiation conditions are presented for control of heparin content (1.6 to 3.3% w/v), modulus (100-10 000 Pa), and gelation time (30-600 s). Encapsulation of 3T3 fibroblasts in the hydrogel gave over 96% viability for all conditions examined. In vitro characterization of epidermal growth factor released from the hydrogel confirmed that the growth factor remains bioactive. The ability to deliver growth factors, fast gelation kinetics under visible light, and independent control of physical and biochemical properties makes this system a promising candidate for use in regenerative medicine. In particular, irradiation conditions that achieve gelation in 150s are compatible with the stringent light exposure limits of the retina, which affords a wide safety margin for use with other tissues.