Improved spiral disk assembly for high-speed counter-current chromatography
Improved spiral disk assembly for high-speed counter-current chromatography
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DOI：
10.1016/j.chroma.2003.08.011
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发表时间：
2003-10-31
影响因子：
4.1
通讯作者：
Ide, D
中科院分区：
文献类型：
--
作者：
Ito, Y;Yang, FQ;Ide, D
对J型高速逆流色谱（HSCCC）螺旋盘进行了改进，在螺旋盘通道中每隔一定距离插入一小段聚四氟乙烯（PTFE）管。最好的结果，得到了一个四通道的螺旋柱，使用600个间隔，显着提高固定相保留和分配效率，在正丁醇-乙酸-水（4：1：5）和聚（乙二醇）1000-磷酸氢二酯聚合物相系统。在此基础上，设计了珠链螺旋盘，并在J型高速离心分离机上进行了性能测试。这种新的色谱柱设计在固定相保留和分配效率方面大大改善了二肽和蛋白质样品的分离。(C)2003 Elsevier B. V保留所有权利。
The spiral disk for type-J high-speed counter-current chromatography (HSCCC) has been improved by placing short segments of PTFE (tetrafluoroethylene) tubing into the channel at regular intervals. The best results were obtained with a four-channel spiral column using 600 spacers, which significantly improved stationary phase retention and partition efficiency in both n-butanol-acetic acid-water (4:1:5) and poly(ethylene glycol) 1000-dibasic phosphate polymer phase systems. Based on these findings, a "bead-chain spiral disk" was designed and its performance tested with a type-J HSCCC centrifuge. This new column design substantially improved the separation of both dipeptide and protein samples in terms of stationary phase retention and partition efficiency. (C) 2003 Elsevier B.V All rights reserved.