SlyA regulates motA and motB, virulence and stress-related genes under conditions induced by the PhoP-PhoQ system in Dickeya dadantii 3937
SlyA regulates motA and motB, virulence and stress-related genes under conditions induced by the PhoP-PhoQ system in Dickeya dadantii 3937
复制标题
DOI：
10.1016/j.resmic.2015.05.004
复制
发表时间：
2015-07-01
影响因子：
2.6
通讯作者：
Tsuyumu, Shinji
中科院分区：
文献类型：
--
作者：
Hague, Md Manjurul;Hirata, Hisae;Tsuyumu, Shinji
我们先前的研究表明Dickeya dadantii 3937的SlyA在对植物的毒力中起着重要的作用，并且Delta SlyA突变体具有高度运动性，而鞭毛的合成和鞭毛蛋白的产生与野生型几乎没有区别。在这里，我们发现，在Delta slyA突变体中，运动性因素，包括连续定向移动的距离、移动的时间和速度，显著高于野生型。值得注意的是，与鞭毛旋转有关的MoTA和MOTB的转录水平在Delta slyA突变体中升高，表明该突变体的过度运动是由于鞭毛旋转的增加。在激活Phop-PhoQ系统的低镁培养基中，Delta slyA突变体的生长和毒力显著低于野生型，MoTA、MOTB、MGTA、PELA、PelB、PELC、PelD、Pele、Pell、Inda、TolC、SODC、ACSA和hrpN的表达也降低。有趣的是，在Phop和PhoQ突变体中，Mota、MOTB、PelD、Pele、Pell、SODC和Inda也减少了。因为SlyA蛋白直接与Phop的启动子区域结合，SlyA通过直接控制多个致病相关基因和/或至少在低镁时通过控制Dadantii 3937中的Phop来调节毒力。(C)2015年巴斯德研究所。爱思唯尔·马森公司出版。版权所有。
We previously showed that SlyA of Dickeya dadantii 3937 plays an important role in virulence toward plants, and that the Delta slyA mutant is hypermotile, whereas flagellum synthesis and flagellin production are indistinguishable from the wild type. Here we show that motility factors, including the distance of continuous directed movement, time for that movement and speed, were significantly higher in the Delta slyA mutant than in the wild type. Remarkably, transcription levels of motA and motB, that are involved in flagellar rotation, were elevated in the Delta slyA mutant, suggesting that the mutant's hypermotility was due to an increase in flagellar rotation. In low (10 mu M) magnesium medium that activates the PhoP-PhoQ system, growth and virulence of the Delta slyA mutant were much lower than for the wild type; expression of motA, motB, mgtA, pelA, pelB, pelC, pelD, pelE, pell, indA, tolC, sodC, acsA and hrpN were also reduced in the mutant. Interestingly, motA, motB, pelD, pelE, pell, sodC and indA were also reduced in phoP and phoQ mutants. Because the SlyA protein directly binds to the promoter region of PhoP, SlyA regulates virulence by controlling multiple pathogenicity-related genes directly and/or at least by controlling PhoP in D. dadantii 3937 when magnesium is low. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.