Imaging RNA Polymerase II transcription sites in living cells
Imaging RNA Polymerase II transcription sites in living cells
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DOI：
10.1016/j.gde.2014.01.002
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发表时间：
2014-04-01
影响因子：
4
通讯作者：
Lis, John T.
中科院分区：
文献类型：
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作者：
Buckley, Martin S.;Lis, John T.
在过去的二十年中，光学和分子成像方法的令人兴奋的发展使研究人员能够以前所未有的分辨率检查细胞核中转录位点的空间组织。从这些研究中发展出来的一个有吸引力的模型是，活性基因聚集在预先形成的转录工厂中，这些转录工厂包含有效的mRNA生物发生所需的多种活性RNA聚合酶和转录因子蛋白。然而，由于转录工厂及其特征仅在固定细胞中被记录，这一模型一直存在广泛的争议。在这篇综述中，我们将重点关注最近的活细胞成像研究，这些研究正在改变我们对转录工厂的理解。
Over the past twenty years, exciting developments in optical and molecular imaging approaches have allowed researchers to examine with unprecedented resolution the spatial organization of transcription sites in the nucleus. An attractive model that has developed from these studies is that active genes cluster to preformed transcription factories that contain multiple active RNA polymerases and transcription factor proteins required for efficient mRNA biogenesis. However, this model has been extensively debated in part due to the fact transcription factories and their features have only been documented in fixed cells. In this review, we will focus on recent live-cell imaging studies that are changing our understanding of transcription factories.