The improvement of M1 polarization in macrophages by glycopeptide derived from Ganoderma lucidum
The improvement of M1 polarization in macrophages by glycopeptide derived from Ganoderma lucidum
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DOI：
10.1007/s12026-017-8893-3
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发表时间：
2017-06-01
影响因子：
4.4
通讯作者：
Duan, Xin-Suo
中科院分区：
文献类型：
--
作者：
Sun, Li-Xin;Lin, Zhi-Bin;Duan, Xin-Suo
灵芝（Fr.）喀斯特（灵芝科）是一种药用蘑菇，数百年来在中国广泛使用，可延年益寿，增强活力，且没有明显的副作用。鉴于灵芝在亚洲以外的其他地区（例如欧洲）最近受到欢迎，人们对灵芝的生物活性特性持续感兴趣。灵芝糖肽（Gl-PS）是从灵芝中分离出的主要有效成分之一。 GL-PS已被证明具有多种生物活性，包括免疫调节和抗肿瘤作用。巨噬细胞是参与先天性和适应性免疫的重要细胞。经典激活的巨噬细胞 (M1) 和替代激活的巨噬细胞 (M2) 具有不同的作用，表现出不同的细胞因子特征：M1 优先产生 TNF-α、IL-6 和 IL-12；M1 优先产生 TNF-α、IL-6 和 IL-12；相反，M2 产生更多的 IL-10 和精氨酸酶。 Gl-PS 可能具有通过脂多糖 (LPS) 促进巨噬细胞 M1 极化的潜力。在本研究中，LPS 用于诱导 M1 极化。结果显示，与对照相比，施用G1-PS后极化的M1巨噬细胞中TNF-α、IL-6和IL-12的水平增加，而IL-10和精氨酸酶I减少。结果表明，G1-PS 具有促进 M1 相对于 M2 极化的潜力，并且了解 G1-PS 的生物活性对健康有益。
Ganoderma lucidum (Fr.) Karst (Ganodermataceae) is a medicinal mushroom that has been extensively used in China for centuries to promote longevity and improve vigor without significant adverse effects. There is continuous interest in the bioactive properties of G. lucidum in view of its newly developed popularity in other regions besides Asia, such as Europe. Glycopeptide derived from G. lucidum (Gl-PS) is one of the main effective components isolated from this mushroom. The Gl-PS has been demonstrated pleiotropic with many bioactivities including immunomodulatory and antitumor effects. Macrophages are important cells involved in innate and adaptive immunity. Classically activated macrophages (M1) and alternatively activated macrophages (M2), with their different roles, display distinct cytokine profiles: M1 preferentially produces TNF-alpha, IL-6, and IL-12; conversely, M2 generates more IL-10 and arginase. Gl-PS might have the potential to promote macrophage M1 polarization by lipopolysaccharide (LPS). In this study, LPS was used to induce the M1 polarization. It was shown that the level of the TNF-alpha, IL-6, and IL-12 were increased and the IL-10 and arginase I were decreased in the polarized M1 macrophages after application of Gl-PS compared to the control. The results indicated the potential of Gl-PS to promote M1 polarization vs M2, with the health beneficial understanding of the bioactivities of Gl-PS.