6-DOF control of single living cells by proximal two-beam optical tweezers
6-DOF control of single living cells by proximal two-beam optical tweezers
复制标题
通过近端双光束光镊对单个活细胞进行六自由度控制
DOI：
10.1117/12.648342
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发表时间：
2005
影响因子：
4
通讯作者：
T. Yasokawa
中科院分区：
文献类型：
--
作者：
M. Yoshida;I. Ishimaru;K. Ishizaki;Y. Inoue;T. Yasokawa
我们对单个活细胞进行了光谱断层扫描研究，以实时获得高空间分辨率的蛋白质的 3 维分布。在本报告中，我们提到了对单个活细胞进行 6 自由度操作以实现高空间分辨率的三维光谱测量。我们提出近端双光束光镊作为旋转操作。我们决定使用两束光束从不同方向照亮每个点的近端两个点。在这种情况下，由光吸收产生的轻压力充当旋转扭矩。使用这种提出的方法，我们可以通过非接触操作来操作微球的旋转速度，而不管折射率分布如何。此外，转速由光学 PWM 操作控制。这种光学 PWM 操作是通过照明时间来改变接收到的光强度。该方法可发展为单细胞的六自由度控制。我们提出了光学空间滤波方法，关注样品产生的衍射光，作为平移速度测量。该测量从随机折射率分布中导出空间频率的任意分量作为周期性光强度分布。该周期性光强度分布根据物体的平移而变化。因此，我们可以通过高响应光电二极管获得未标记细胞的平移速度。
We performed a spectroscopy-tomography study of a single living cell to obtain 3-dimensional distribution of proteins in high spatial resolution in real time. In this report, we mention the 6-DOF manipulation of a single living cell to achieve the high spatial resolution 3-dimentional spectrometry. We propose the proximal two- beam optical tweezers as rotational operation. We decided to illuminate the proximal two points in each from different directions using two beams. In this case, the light pressure generated by light absorption is made to act as rotating torque. Using this proposed method, we can operate the rotational velocity of a microsphere regardless of refractive index distribution by non-contact operation. In addition, rotational speed is controlled by optical PWM operation. This proposed optical PWM operation is that the received light intensity is changed by the illumination time. This method can be developed into the 6-DOF control of single-cell. And we propose the optical spatial filtering method, paying attention to the diffracted light that is generated from a sample, as translational velocity measurement. This measurement derives the arbitrary component of the spatial frequency from the random refracted index distribution as the periodic light intensity distribution. This periodic light intensity distribution changes in accordance with the translation of an object. Therefore, we can obtain the translational velocity of the non- labeled cell by high-response photodiode.