Urinary screening for alprazolam, triazolam, and their metabolites with the EMIT d.a.u. benzodiazepine metabolite assay.
Urinary screening for alprazolam, triazolam, and their metabolites with the EMIT d.a.u. benzodiazepine metabolite assay.
复制标题
使用 EMIT d.a.u. 尿液筛查阿普唑仑、三唑仑及其代谢物。
DOI：
10.1093/jat/11.6.263
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发表时间：
1987
影响因子：
2.5
通讯作者：
A. Fraser
中科院分区：
文献类型：
--
作者：
A. Fraser
阿普唑仑（Xanax）和三唑仑（Halcion）是相对较新的三唑苯并二氮卓类药物，具有抗焦虑和催眠作用。本研究评估了EMIT d.a.u.中这些药物及其主要代谢产物的反应性。苯二氮卓代谢物测定。采用EMIT分析药物和代谢物的分析标准品以及接受这些药物的患者的尿液标本。阿普唑仑和α-OH阿普唑仑对EMIT低校准品的响应在0.2和0.3 μ g/mL之间相当。三唑仑和α-OH三唑仑在0.3和0.5 μ g/mL之间具有反应性。阿普唑仑治疗患者的27份随机尿液标本中有24份检测呈阳性，三唑仑治疗患者的19份尿液标本中有8份检测呈阳性。通过HPLC法测定主要尿液代谢产物α-OH阿普唑仑和α-OH三唑仑，证实尿液结果呈阳性。该研究表明，EMIT测定法可以检测尿液中大量的阿普唑仑和代谢物。然而，在接受三唑仑治疗的患者中，58%的样本检测结果为阴性。
Alprazolam (Xanax) and triazolam (Halcion) are relatively new triazolobenzodiazepines that are anxiolytic and hypnotic. This study assesses the reactivity of these drugs and their major metabolites in the EMIT d.a.u. benzodiazepine metabolite assay. Analytical standards of drugs and metabolites and urine specimens from patients receiving these drugs were analyzed by EMIT. Alprazolam and alpha-OH alprazolam gave an equivalent response to the EMIT low calibrator between 0.2 and 0.3 microgram/mL. Triazolam and alpha-OH triazolam were reactive between 0.3 and 0.5 microgram/mL. The assay was positive in 24 out of 27 random urine specimens from alprazolam-treated patients and in 8 out of 19 urine specimens from triazolam-treated patients. Positive urine results were confirmed by measuring the major urinary metabolites alpha-OH alprazolam and alpha-OH triazolam by HPLC. The study demonstrates that the EMIT assay can detect significant amounts of alprazolam and metabolites in the urine. The assay was negative in 58% of the specimens from individuals receiving triazolam, however.