Photo-cross-linked decyl methacrylate films for electrochemical and optical polyion probes.
Photo-cross-linked decyl methacrylate films for electrochemical and optical polyion probes.
复制标题
用于电化学和光学聚离子探针的光交联甲基丙烯酸癸酯薄膜。
DOI：
10.1021/ac9703459
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发表时间：
1997
影响因子：
7.4
通讯作者：
Meyerhoff,ME
中科院分区：
文献类型：
--
作者：
Ambrose,TM;Meyerhoff,ME
基于光交联甲基丙烯酸癸酯（DMA）薄膜的电位和光学聚离子探针进行了描述，并详细研究了膜组合物对肝素响应的影响。根据现有的理论管理电位聚离子响应，降低膜内的增塑剂和三十二烷基甲基氯化铵离子交换剂的量，提高其对肝素的敏感性。然而，改变基于DMA的膜的交联剂含量提供了调节其物理结构的额外机制，并因此提供了观察到的电位聚离子响应。具有低己二醇二甲基丙烯酸酯交联剂含量的膜产生最佳的电位肝素检测限（0.04 μM），表明在这种膜内的扩散系数较低，这显然是由于相邻的侧基癸基之间的相互作用。增加交联剂含量会中断这些相互作用并促进扩散。这方面的知识被应用于优化光学肝素传感通过DMA薄膜共价连接到玻璃基板。当在有限体积/固定暴露时间测量模式下使用时，这种光学敏感膜可以检测未稀释的人血浆中临床相关水平的肝素（0.5 - 5.0单位/mL）。
Potentiometric and optical polyion probes based on photo-cross-linked thin films of decyl methacrylate (DMA) are described, and the effects of film composition on the response toward heparin are examined in detail. In accordance with existing theory governing potentiometric polyion response, lowering the amounts of plasticizer and tridodecylmethylammonium chloride ion exchanger within the film enhances its sensitivity toward heparin. Varying the cross-linker content of a DMA-based film, however, provides an additional mechanism to regulate its physical structure and, hence, the observed potentiometric polyion response. Films with low hexanedioldimethacrylate cross-linker content yield optimal potentiometric heparin detection limits (0.04 μM), suggesting a lower diffusion coefficient within such films, apparently due to interactions between adjacent pendant decyl groups. Increasing cross-linker content interrupts these interactions and facilitates diffusion. This knowledge is applied to optimize optical heparin sensing via DMA films covalently attached to glass substrates. When used in a limited volume/fixed exposure time measurement mode, such optically sensitive films can detect clinically relevant levels of heparin (0.5−5.0 units/mL) in undiluted human plasma.