THE TRIMETHYLGUANOSINE CAP STRUCTURE OF U1 SNRNA IS A COMPONENT OF A BIPARTITE NUCLEAR TARGETING SIGNAL
THE TRIMETHYLGUANOSINE CAP STRUCTURE OF U1 SNRNA IS A COMPONENT OF A BIPARTITE NUCLEAR TARGETING SIGNAL
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DOI：
10.1016/0092-8674(90)90021-6
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发表时间：
1990-08-10
期刊：
影响因子：
64.5
通讯作者：
MATTAJ, IW
中科院分区：
文献类型：
--
作者：
HAMM, J;DARZYNKIEWICZ, E;MATTAJ, IW
分析了一系列U1 snRNA和U6 snRNA在注入非洲爪蟾卵母细胞的细胞质后迁移到细胞核中的能力。通过将U snRNA基因注射到卵母细胞的核中或通过T7 RNA聚合酶合成，结合各种帽结构来制备U snRNA。结果表明，核靶向U1 snRNA需要一个三甲基鸟苷帽结构和至少一个共同的U snRNP蛋白的结合。使用合成的U6 snRNA，进一步证明了三甲基鸟苷帽结构可以在不存在常见的U snRNP蛋白的情况下在核靶向中起作用。这些结果表明，U snRNP核靶向信号是一个模块的性质。
The ability of a series of U1 snRNAs and U6 snRNAs to migrate into the nucleus of Xenopus oocytes after injection into the cytoplasm was analyzed. The U snRNAs were made either by injecting U snRNA genes into the nucleus of oocytes or, synthetically, by T7 RNA polymerase, incorporating a variety of cap structures. The results indicate that nuclear targeting of U1 snRNA requires both a trimethylguanosine cap structure and binding of at least one common U snRNP protein. Using synthetic U6 snRNAs, it is further demonstrated that the trimethylguanosine cap structure can act in nuclear targeting in the absence of the common U snRNP proteins. These results imply that U snRNP nuclear targeting signals are of a modular nature.