A new database of mitochondrial DNA hypervariable regions I and II sequences from 162 Japanese individuals
A new database of mitochondrial DNA hypervariable regions I and II sequences from 162 Japanese individuals
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DOI：
10.1007/s004140100211
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发表时间：
2002-04-01
影响因子：
2.1
通讯作者：
Holland, MM
中科院分区：
文献类型：
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作者：
Imaizumi, K;Parsons, TJ;Holland, MM
从162个无关的日本个体中建立了线粒体DNA（mtDNA）高变区1（HV 1）和控制区2（HV 2）序列数据库。该数据库的随机匹配概率和遗传多样性分别为0.96%和0.997。分别在37%和38%的样品中观察到HV 1中位于位置16189和HV 2中位于位置310周围的C-伸展区域中的长度异质性。设计了使用内部测序引物的策略，以在这些长度异质性个体中获得确认的序列。这个数据库，结合其他mtDNA序列数据库从日本人口，将允许mtDNA匹配结果的意义，在日本的mtDNA分型个案工作中得到适当的报告。
A database of mitochondrial DNA (mtDNA) hypervariable region 1 (HV1) and region 2 (HV2) sequences of the mtDNA control region was established from 162 unrelated Japanese individuals. The random match probability and the genetic diversity for this database were 0.96% and 0.997, respectively. Length heteroplasmy in the C-stretch regions located around position 16189 in HV1 and 310 in HV2 was observed in 37% and 38% of the samples, respectively. A strategy using internal sequencing primers was devised to obtain confirmed sequences in these length heteroplasmic individuals. This database, combined with other mtDNA sequence databases from the Japanese population, will permit the significance of mtDNA match results to be properly reported in mtDNA typing casework in Japan.