Raman Activated Cell Ejection for Isolation of Single Cells
Raman Activated Cell Ejection for Isolation of Single Cells
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DOI：
10.1021/ac403107p
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发表时间：
2013-11-19
影响因子：
7.4
通讯作者：
Huang, Wei E.
中科院分区：
文献类型：
--
作者：
Wang, Yun;Ji, Yuetong;Huang, Wei E.
我们已经优化了一个拉曼显微镜，以获得单细胞拉曼光谱（SCRS）与0.1秒的采集时间。具有如此短的采集时间的SCRS具有足够的区分能力和光谱再现性，以区分掺入C-13和N-15的细胞，并对从口腔中分离的五种不同类型的细菌进行分类。我们还开发了拉曼激活的细胞喷射（RACE），这是由激光诱导的前向转移（LIFT）的辅助。我们首次证明，可以识别感兴趣的单细胞，然后根据它们的SCRS从复杂的微生物群落中准确分离。该方法可用于从复杂样品中分选目标性状的单细胞（例如，生物膜、土壤、污泥、组织）。
We have optimized a Raman microscope to obtain a single cell Raman spectrum (SCRS) with 0.1 s acquisition time. SCRS with such short acquisition time has sufficient discriminatory ability and spectral reproducibility to differentiate cells incorporated with C-13 and N-15 and to classify five different types of bacteria isolated from the oral cavity. We also developed Raman activated cell ejection (RACE) that is assisted by laser induced forward transfer (LIFT). We have shown, for the first time, that the single cells of interest can be identified and then accurately isolated from complex microbial communities based on their SCRS. This approach can be used to sort single cells of target traits from complex samples (e.g., biofilms, soils, sludge, tissues).