Splice junctions are constrained by protein disorder.
Splice junctions are constrained by protein disorder.
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DOI：
10.1093/nar/gkv407
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发表时间：
2015-05-26
影响因子：
14.9
通讯作者：
Gough J
中科院分区：
文献类型：
--
作者：
Smithers B;Oates ME;Gough J
我们已经发现，基因中剪接连接的位置受到翻译蛋白质区域对促进无序的氨基酸的耐受性的限制。众所周知，有效的剪接需要剪接连接处的核苷酸偏向；首选的用法是产生促进无序的氨基酸分布。我们观察到，在氨基酸分布中，剪接的效率并没有因适应球状结构而受到影响。因此，我们推断，剪接连接在基因中的位置必须受到局部蛋白质环境的限制。检测在基因剪接连接附近发现的外显子剪接增强子，发现这些(短DNA基序)在编码无序蛋白质区域的外显子中比编码结构区域的外显子中更普遍。因此，我们还得出结论，局部蛋白质特征在结构上比在无序上更多地限制了有效的剪接。
We have discovered that positions of splice junctions in genes are constrained by the tolerance for disorder-promoting amino acids in the translated protein region. It is known that efficient splicing requires nucleotide bias at the splice junction; the preferred usage produces a distribution of amino acids that is disorder-promoting. We observe that efficiency of splicing, as seen in the amino-acid distribution, is not compromised to accommodate globular structure. Thus we infer that it is the positions of splice junctions in the gene that must be under constraint by the local protein environment. Examining exonic splicing enhancers found near the splice junction in the gene, reveals that these (short DNA motifs) are more prevalent in exons that encode disordered protein regions than exons encoding structured regions. Thus we also conclude that local protein features constrain efficient splicing more in structure than in disorder.
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DOI：
10.1016/j.bbapap.2010.01.017
发表时间：
2010-06
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Uversky VN;Dunker AK
通讯作者：
Dunker AK
影响因子：
14.9
作者：
Flicek P;Ahmed I;Amode MR;Barrell D;Beal K;Brent S;Carvalho-Silva D;Clapham P;Coates G;Fairley S;Fitzgerald S;Gil L;García-Girón C;Gordon L;Hourlier T;Hunt S;Juettemann T;Kähäri AK;Keenan S;Komorowska M;Kulesha E;Longden I;Maurel T;McLaren WM;Muffato M;Nag R;Overduin B;Pignatelli M;Pritchard B;Pritchard E;Riat HS;Ritchie GR;Ruffier M;Schuster M;Sheppard D;Sobral D;Taylor K;Thormann A;Trevanion S;White S;Wilder SP;Aken BL;Birney E;Cunningham F;Dunham I;Harrow J;Herrero J;Hubbard TJ;Johnson N;Kinsella R;Parker A;Spudich G;Yates A;Zadissa A;Searle SM
通讯作者：
Searle SM
影响因子：
9.8
作者：
Parmley JL;Urrutia AO;Potrzebowski L;Kaessmann H;Hurst LD
通讯作者：
Hurst LD
影响因子：
12.3
作者：
Cáceres EF;Hurst LD
通讯作者：
Hurst LD
影响因子：
13.8
作者：
Blencowe, BJ
通讯作者：
Blencowe, BJ