Costs of insensitive acetylcholinesterase insecticide resistance for the malaria vector Anopheles gambiae homozygous for the G119S mutation
Costs of insensitive acetylcholinesterase insecticide resistance for the malaria vector Anopheles gambiae homozygous for the G119S mutation
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DOI：
10.1186/1475-2875-9-12
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发表时间：
2010-01-13
期刊：
影响因子：
3
通讯作者：
Agnew, Philip
中科院分区：
文献类型：
--
作者：
Djogbenou, Luc;Noel, Valerie;Agnew, Philip
背景：最近在西非冈比亚按蚊自然种群中报道了导致乙酰胆碱酯酶对有机磷和氨基甲酸酯杀虫剂不敏感的抗性的 G119S 突变。这些报告表明，An 的这种突变会产生耐药性成本。冈比亚，特别是对于纯合个体，这些成本可能会影响确定这些群体中氨基甲酸酯抗性的频率。方法：AcerKis 和 Kisumu 菌株的生活史特征。在无杀虫剂的实验室环境中进行的三个独立实验中，对幼虫食物供应情况进行了比较。这两个品系具有相同的遗传背景，但其纯合性不同，分别在于ace-1位点是否存在G119S突变。结果：抗性品系的蛹比敏感品系的蛹在化蛹期间死亡的可能性明显更高。抗性品系的化蛹年龄明显更早，干饥饿重量也明显更轻；当两个品系的化蛹年龄相匹配时，这种体重差异仍然存在。结论：An. 发现的抗性的主要成本。冈比亚蚊子的 G119S 突变纯合子的一个特点是，它们在化蛹期间比易感蚊子更容易死亡，而且它们在一系列幼虫食物条件下都是如此。比较来自同一种群的第四龄幼虫和成虫中 G119S 的频率将提供一种方法来测试这种抵抗成本是否在按蚊的自然种群中表达。冈比亚并影响这种抗性突变的动态。
Background: The G119S mutation responsible for insensitive acetylcholinesterase resistance to organophosphate and carbamate insecticides has recently been reported from natural populations of Anopheles gambiae in West Africa. These reports suggest there are costs of resistance associated with this mutation for An. gambiae, especially for homozygous individuals, and these costs could be influential in determining the frequency of carbamate resistance in these populations.Methods: Life-history traits of the AcerKis and Kisumu strains of An. gambiae were compared following the manipulation of larval food availability in three separate experiments conducted in an insecticide-free laboratory environment. These two strains share the same genetic background, but differ in being homozygous for the presence or absence of the G119S mutation at the ace-1 locus, respectively.Results: Pupae of the resistant strain were significantly more likely to die during pupation than those of the susceptible strain. Ages at pupation were significantly earlier for the resistant strain and their dry starved weights were significantly lighter; this difference in weight remained when the two strains were matched for ages at pupation.Conclusions: The main cost of resistance found for An. gambiae mosquitoes homozygous for the G119S mutation was that they were significantly more likely to die during pupation than their susceptible counterparts, and they did so across a range of larval food conditions. Comparing the frequency of G119S in fourth instar larvae and adults emerging from the same populations would provide a way to test whether this cost of resistance is being expressed in natural populations of An. gambiae and influencing the dynamics of this resistance mutation.