Atomic model of vesicular stomatitis virus and mechanism of assembly.
Atomic model of vesicular stomatitis virus and mechanism of assembly.
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DOI：
10.1038/s41467-022-33664-4
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发表时间：
2022-10-10
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
水泡性口炎病毒（VSV）与其他负链RNA病毒（NSV）（如流感病毒和狂犬病病毒）一样，具有三层结构：一层基质蛋白（M）位于布满糖蛋白（G）的膜囊和核衣壳之间，由核衣壳蛋白（N）和核衣壳化的基因组RNA组成。缺乏这些病毒组分的原位原子结构限制了对组装子弹形病毒体的机械理解。在这里，通过cryoEM和亚粒子重建，我们已经确定了在3.47纳米分辨率的VSV内部的M和N的原位结构。在病毒体中，N和M位点具有1：2的化学计量。N和M的原位结构与它们的晶体结构在它们的N-末端片段和寡聚环中不同。N-RNA、N-N和N-M-M相互作用控制衣壳的形成。双层M有助于螺旋核衣壳的包装：内部M（IM）连接N螺旋的相邻匝，而外部M（OM）接触G和膜包膜。G的赝晶组织通过cryoET进一步映射。VSV组装的机制是由这些病毒组分的网络相互作用来描述的。Zhou和Si等人使用低温电子显微镜和断层扫描技术描绘了水泡性口炎病毒基因组RNA、核衣壳蛋白、基质蛋白和糖蛋白之间的分子相互作用，并提出了一种组装模型。
Like other negative-strand RNA viruses (NSVs) such as influenza and rabies, vesicular stomatitis virus (VSV) has a three-layered organization: a layer of matrix protein (M) resides between the glycoprotein (G)-studded membrane envelope and the nucleocapsid, which is composed of the nucleocapsid protein (N) and the encapsidated genomic RNA. Lack of in situ atomic structures of these viral components has limited mechanistic understanding of assembling the bullet-shaped virion. Here, by cryoEM and sub-particle reconstruction, we have determined the in situ structures of M and N inside VSV at 3.47 Å resolution. In the virion, N and M sites have a stoichiometry of 1:2. The in situ structures of both N and M differ from their crystal structures in their N-terminal segments and oligomerization loops. N-RNA, N-N, and N-M-M interactions govern the formation of the capsid. A double layer of M contributes to packaging of the helical nucleocapsid: the inner M (IM) joins neighboring turns of the N helix, while the outer M (OM) contacts G and the membrane envelope. The pseudo-crystalline organization of G is further mapped by cryoET. The mechanism of VSV assembly is delineated by the network interactions of these viral components. Zhou and Si et al. used cryogenic electron microscopy and tomography to delineate the molecular interactions among genomic RNA, nucleocapsid protein, matrix protein and glycoprotein in vesicular stomatitis virus and suggest a model of assembly.
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影响因子：
16.6
作者：
通讯作者：
--
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
3.7
作者：
GAUDIN, Y;BARGE, A;RUIGROK, RWH
通讯作者：
RUIGROK, RWH
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH