Coexpression of ERβ with ERα and progestin receptor proteins in the female rat forebrain:: Effects of estradiol treatment
Coexpression of ERβ with ERα and progestin receptor proteins in the female rat forebrain:: Effects of estradiol treatment
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DOI：
10.1210/en.142.12.5172
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发表时间：
2001-12-01
期刊：
影响因子：
4.8
通讯作者：
Blaustein, JD
中科院分区：
文献类型：
--
作者：
Gréco, B;Allegretto, EA;Blaustein, JD
雌激素和孕激素受体（ER、PgR）在女性神经内分泌功能的调节中起着重要作用。最近克隆的ER β的神经解剖学分布与Ella和PgR重叠。为了确定雌鼠中ER β是否存在于ER α或PgR神经元中，我们使用双标记免疫细胞化学。ER β-免疫反应（ER β-ir）主要在室周视前区（PvPO）、终纹床核（BNSTpr）、室旁核、视上核和内侧杏仁核（MEApd）的细胞核中检测到。在去卵巢大鼠的PvPO、BNSTpr和MEApd中观察到ER β-ir与ER α-ir或PgR-ir的共表达。E2处理减少了PvPO和BNSTpr中ER β-ir细胞的数量以及MEApd和室旁核中ER α-ir细胞的数量，从而减少了PvPO、BNSTpr和MEApd中共表达ER β-ir和ER α-ir的细胞的数量。E2处理增加了PvPO、BNSTpr和MEApd细胞中PgR-ir的量，其中一部分还含有ER β。这些结果表明，ER β表达在ER α或PgR-含有细胞，他们建议，E可以调节这些类固醇受体的比例，在大脑区域特异性的方式。
Estrogen and progestin receptors (ER, PgR) play a critical role in the regulation of neuroendocrine functions in females. The neuroanatomical distribution of the recently cloned, ER beta, overlaps with both Ella and PgR. To determine whether ER beta is found within ER alpha- or PgR-containing neurons in female rat, we used dual label immunocytochemistry. ER beta -immunoreactivity (ER beta -ir) was primarily detected in the nuclei of cells in the periventricular preoptic area (PvPO), the bed nucleus of the stria terminalis (BNSTpr), the paraventricular nucleus, the supraoptic nucleus, and the medial amygdala (MEApd). Coexpression of ER beta -ir with ER alpha -ir or PgR-ir was observed in the PvPO, BNSTpr, and MEApd in ovariectomized rats. E2 treatment decreased the number of ER beta -ir cells in the PvPO and BNSTpr and the number of ER alpha -ir cells in the MEApd and paraventricular nucleus, and therefore decreased the number of cells coexpressing ER beta -ir and ER alpha -ir in the PvPO, BNSTpr, and MEApd. E2 treatment increased the amount of PgR-ir in cells of the PvPO, BNSTpr, and MEApd, a portion of which also contained ER beta. These results demonstrate that ER beta is expressed in ER alpha- or PgR-containing cells, and they suggest that E can modulate the ratios of these steroid receptors in a brain region-specific manner.