Analysis of aldehydes in beer using solid-phase microextraction with on-fiber derivatization and gas chromatography/mass spectrometry.
Analysis of aldehydes in beer using solid-phase microextraction with on-fiber derivatization and gas chromatography/mass spectrometry.
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DOI：
10.1021/jf034410t
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发表时间：
2003-10
影响因子：
6.1
通讯作者：
P. Veselý;Lance T. Lusk;G. Basařová;J. Seabrooks;D. Ryder
中科院分区：
文献类型：
--
作者：
P. Veselý;Lance T. Lusk;G. Basařová;J. Seabrooks;D. Ryder
建立了一种新的快速、灵敏、无溶剂提取技术来分析啤酒中的羰基化合物。该方法基于固相微萃取-纤维上衍生化。衍生化试剂O-(2，3，4，5，6-五氟苯基)羟胺(PFBOA)被吸附在二乙烯基苯/聚(二甲基硅氧烷)65微米纤维上，暴露在装有啤酒样品的小瓶的顶空中。羰基化合物选择性地与PFBOA反应，生成的肟被解吸到气相色谱进样口并用质谱仪进行定量。该方法具有很高的重现性和线性。用于陈化啤酒的分析，检出9种醛：2-甲基丙醛、2-甲基丁醛、3-甲基丁醛、正戊醛、己醛、呋喃甲醛、甲醛、苯乙醛和(E)-2-壬烯醛。
A new, fast, sensitive, and solventless extraction technique was developed in order to analyze beer carbonyl compounds. The method was based on solid-phase microextraction with on-fiber derivatization. A derivatization agent, O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBOA), was absorbed onto a divinyl benzene/poly(dimethylsiloxane) 65-microm fiber and exposed to the headspace of a vial with a beer sample. Carbonyl compounds selectively reacted with PFBOA, and the oximes formed were desorbed into a gas chromatograph injection port and quantified by mass spectrometry. This method provided very high reproducibility and linearity. When it was used for the analysis of aged beers, nine aldehydes were detected: 2-methylpropanal, 2-methylbutanal, 3-methylbutanal, pentanal, hexanal, furfural, methional, phenylacetaldehyde, and (E)-2-nonenal.