An efficient quantitation method of next-generation sequencing libraries by using MiSeq sequencer
An efficient quantitation method of next-generation sequencing libraries by using MiSeq sequencer
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DOI：
10.1016/j.ab.2014.08.015
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发表时间：
2014-12-01
影响因子：
2.9
通讯作者：
Yamamoto, Masayuki
中科院分区：
文献类型：
--
作者：
Katsuoka, Fumiki;Yokozawa, Junji;Yamamoto, Masayuki
文库定量是Illumina HiSeq测序仪获得高数据输出的关键步骤。在这里，我们介绍了一种文库定量方法，该方法使用Illumina MiSeq测序仪，称为定量MiSeq （qMiSeq）。在这个过程中，96个双索引文库，包括对照样本，变性，池在等体积，并通过MiSeq测序。我们发现，每个文库的相对浓度可以根据观察到的指数比来确定，并可以用来确定每个文库的HiSeq运行条件。因此，qMiSeq提供了一次定量大量库的有效方法。(C) 2014爱思唯尔公司版权所有。
Library quantitation is a critical step to obtain high data output in Illumina HiSeq sequencers. Here, we introduce a library quantitation method that uses the Illumina MiSeq sequencer designated as quantitative MiSeq (qMiSeq). In this procedure, 96 dual-index libraries, including control samples, are denatured, pooled in equal volume, and sequenced by MiSeq. We found that relative concentration of each library can be determined based on the observed index ratio and can be used to determine HiSeq run condition for each library. Thus, qMiSeq provides an efficient way to quantitate a large number of libraries at a time. (C) 2014 Elsevier Inc. All rights reserved.