CRISPR/Cas9 in yeast: a multi-week laboratory exercise for undergraduate students
CRISPR/Cas9 in yeast: a multi-week laboratory exercise for undergraduate students
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DOI：
10.24918/cs.2019.19
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发表时间：
2019
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通讯作者：
R. Ulbricht
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作者：
R. Ulbricht
为本科生命科学专业的学生提供基于课程的研究经验，利用尖端技术，对学生来说是易于驾驭的，并且作为教师是可管理的，这是一个挑战。在这里，我描述了一个多周的课程计划，以编辑芽殖酵母，酿酒酵母的基因组为目标的实验室为基础的课程。学生应用有关高级主题的知识，如：CRISPR/Cas9基因编辑，DNA修复，遗传学和克隆。本课要求学生掌握生物信息学分析、限制性内切酶消化、连接、微生物学基本技能、聚合酶链反应、质粒纯化等技能。指导教师通过协议的技术方面，以及整个实验室经验涉及的教学理念。就目前而言，实验室课程适合于6-8周的高水平本科实验室课程，但可以适应较短的工作时间和经验较少的学生。学生完成课程与生命科学研究的更现实的想法，并报告显着的学习收获。我希望这一课能为本科生课程的教师和学生提供一个实践性的、以发现为基础的学习体验，让学生培养在生命科学领域取得成功所必需的技能。引文：Ulbricht，R. J. 2019。酵母中的CRISPR/Cas9：针对本科生的多周实验室练习CourseSource. https://doi.org/10.24918/cs.2019.19编辑：Jennifer Knight，University of科罗拉多博尔德接收：10/26/2018;接受：02/12/2019;出版：05/31/2019版权：© 2019 Ulbricht.这是一篇开放获取的文章，根据知识共享署名-非商业性-相同方式共享4.0国际许可证的条款分发，该许可证允许在任何媒体上不受限制的非商业性使用，分发和复制，前提是原作者和来源被记入。利益冲突和资金声明：这项工作是由密苏里州州立大学的生物医学科学系支持。作者没有与这项工作有关的财务，个人或专业利益冲突。支持材料：S1。CRISPR in Yeast Lecture; S2.酵母样品中的CRISPR评估; S3. CRISPR in Yeast Laboratory Manual; S4. CRISPR in Yeast教师笔记; S5.酵母中的CRISPR实验室报告标题;和S6.酵母数据分析中的CRISPR。地址：901 South National Avenue Springfield，MO 65897办公室电话：417-836-5730电子邮件：RandiUlbricht@MissouriState.edu CourseSource|www.coursesource.org 2019|第06卷第1课
Providing undergraduate life-science students with a course-based research experience that utilizes cutting-edge technology, is tractable for students, and is manageable as an instructor is a challenge. Here, I describe a multi-week lesson plan for a laboratory-based course with the goal of editing the genome of budding yeast, Saccharomyces cerevisiae. Students apply knowledge regarding advanced topics such as: CRISPR/Cas9 gene editing, DNA repair, genetics, and cloning. The lesson requires students to master skills such as bioinformatics analysis, restriction enzyme digestion, ligation, basic microbiology skills, polymerase chain reaction, and plasmid purification. Instructors are led through the technical aspects of the protocols, as well as the teaching philosophy involved throughout the laboratory experience. As it stands, the laboratory lesson is appropriate for 6-8 weeks of an upper-level undergraduate laboratory course, but may be adapted for shorter stints and students with less experience. Students complete the lesson with a more realistic idea of life science research and report significant learning gains. I anticipate this lesson to provide instructors and students in undergraduate programs with a hands-on, discovery-based learning experience that allows students to cultivate skills essential for success in the life sciences. Citation: Ulbricht, R.J. 2019. CRISPR/Cas9 in yeast: a multi-week laboratory exercise for undergraduate students. CourseSource. https://doi.org/10.24918/cs.2019.19 Editor: Jennifer Knight, University of Colorado Boulder Received: 10/26/2018; Accepted: 02/12/2019; Published: 05/31/2019 Copyright: © 2019 Ulbricht. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. Conflict of Interest and Funding Statement: This work is supported by the Biomedical Sciences Department at Missouri State University. The author does not have a financial, personal, or professional conflict of interest related to this work. Supporting Materials: S1. CRISPR in Yeast Lecture; S2. CRISPR in Yeast Sample Assessments; S3. CRISPR in Yeast Laboratory Manual; S4. CRISPR in Yeast Instructor Notes; S5. CRISPR in Yeast Lab Report Rubric; and S6. CRISPR in Yeast Data Analysis. *Correspondence to: 901 South National Avenue Springfield, MO 65897. Office phone: 417-836-5730 Email: RandiUlbricht@MissouriState.edu CourseSource | www.coursesource.org 2019 | Volume 06 1 Lesson