Effect of taurine on a muscle intracellular membrane.
Effect of taurine on a muscle intracellular membrane.
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DOI：
10.1016/0005-2736(73)90165-x
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发表时间：
1973-11
期刊：
影响因子：
--
通讯作者：
R. Huxtable;R. Bressler
中科院分区：
文献类型：
--
作者：
R. Huxtable;R. Bressler
从大鼠骨骼肌肌浆网草酸钙摄取率依赖于肌肉被删除和均质化的速度。快速分离的肌浆网（肌肉去除和均质化20分钟内）表现出25%的草酸钙的摄取率在15 mM牛磺酸的存在下增加，和总的螯合能力也增加。与不含牛磺酸的成对分离相比，通过在所有培养基中使用10-15 mM牛磺酸进行分离，可以使更缓慢分离的肌浆网（第一只动物处死和肌肉均质化之间1 h）的草酸钙摄取速率增加30%。在整个隔离过程中，牛磺酸暴露于肌浆网导致微粒体和肌浆网产量增加。牛磺酸对肌浆网的这些作用不被磷酸盐、羟乙基磺酸盐、KCl、半胱氨酸或组氨酸复制，但氨基丙磺酸显示出类似的作用。牛磺酸可减缓磷脂酶C引起的肌浆网钙转运和ATP酶活性的丧失。这表明牛磺酸可能作为一种膜稳定剂。
Sarcoplasmic reticulum from rat skeletal muscle had rates of calcium oxalate uptake dependent upon the speed with which the muscle was removed and homogenized. Rapidly isolated sarcoplasmic reticulum (muscle removed and homogenized within 20 min) showed a 25% increase in rate of calcium oxlate uptake in the presence of 15 mM taurine, and the total sequestering capacity was also increased. The rate of calcium oxalate uptake by more slowly isolated sarcoplasmic reticulum (1 h between sacrifice of first animal and homogenization of the muscle) could be increased by 30% by performing the isolation with 10–15 mM taurine present in all media, compared with a paired isolation in the absence of taurine. Exposure of sarcoplasmic reticulum to taurine throughout isolation led to an increased yield of microsomes and sarcoplasmic reticulum. These effects of taurine on sarcoplasmic reticulum were not duplicated by phosphate, isethionate, KCl, cysteine or histidine, but aminopropane-sulfonic acid showed similar effects. Taurine slowed the rate of loss of calcium transport and ATPase activities of sarcoplasmic reticulum caused by phospholipase C. It is suggested that taurine may function as a membrane stabilizer.