Effects of ethanol and acetaldehyde on the biosynthesis of testosterone in the rodent testes.
Effects of ethanol and acetaldehyde on the biosynthesis of testosterone in the rodent testes.
复制标题
乙醇和乙醛对啮齿动物睾丸睾酮生物合成的影响。
DOI：
10.1016/0006-291x(80)91307-8
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
R. D. Bell
中科院分区：
文献类型：
--
作者：
T. Cicero;R. D. Bell
在酶分散的啮齿动物睾丸细胞中，研究了乙醇和乙醛对睾丸类固醇合成的影响。这两种药物都显著抑制促性腺激素刺激的类固醇生成，但乙醛的效力比乙醇强得多(>；1000倍)。为了确定这两种药物抑制睾酮生物合成途径中的哪一步，将细胞与[~3H]孕烯醇酮和[~3H]孕酮共同孵育，并测定其与睾酮及其前体的标记掺入量。乙醇和乙醛只抑制雄烯二酮向睾酮的转化；其他睾酮前体不受影响。
The effects of ethanol and acetaldehyde on testicular steroidogenesis were examined in enzymatically dispersed cells of the rodent testes. Both drugs significantly inhibited gonadotropin-stimulated steroidogenesis, but acetaldehyde was considerably more potent (>1000 times) than ethanol. To determine the step in testosterone's biosynthetic pathway which was inhibited by the two drugs, cells were incubated in the presence of [3H]pregnenolone and [3H]progesterone, and the amount of label incorporated into testosterone and its precursors was determined. Ethanol and acetaldehyde inhibited only the conversion of androstenedione to testosterone; none of the other precursors of testosterone was affected.