Terpinen-4-ol Enhances Disease Resistance of Postharvest Strawberry Fruit More Effectively than Tea Tree Oil by Activating the Phenylpropanoid Metabolism Pathway
Terpinen-4-ol Enhances Disease Resistance of Postharvest Strawberry Fruit More Effectively than Tea Tree Oil by Activating the Phenylpropanoid Metabolism Pathway
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DOI：
10.1021/acs.jafc.0c01840
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发表时间：
2020-06-17
影响因子：
6.1
通讯作者：
Shao, Xingfeng
中科院分区：
文献类型：
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作者：
Li, Zhenbiao;Wang, Nan;Shao, Xingfeng
本研究旨在揭示茶树油（TTO）的主要成分松油烯-4-醇对草莓果实抗病性的影响及其可能的机制。结果表明，用松油烯-4-醇处理的草莓在48h后发病率最低（44.4%），整个贮藏过程中病害直径最小。这表明，与TTO和其他成分相比，松油烯-4-醇对草莓的抗病性最强。非靶向代谢组学分析表明，在处理后12 h，松油烯-4-醇处理通过增加果实中肉桂醛、松柏醛、柚皮素、杉木素、槲皮素和槲皮素的积累，强烈激活了苯丙素生物合成和类黄酮代谢途径。此外，松油烯-4-醇处理还通过增强苯丙素代谢途径中关键酶的活性和相关基因表达，引起总酚和木质素的积累。上述结果表明，松油烯-4-醇是TTO的关键成分，是TTO通过激活苯丙素代谢途径提高草莓果实抗病性的最重要因素。
This study aimed to reveal the effects and possible mechanism of terpinen-4-ol, the main component of tea tree oil (TTO), on the disease resistance of strawberry fruit. When the effects of TTO and its components were compared on the decay development in fruit inoculated with Botrytis cinerea after treatment, strawberry treated with terpinen-4-ol showed the lowest disease incidence (44.4%) after 48 h and also the smallest lesion diameter during the whole storage. This indicates that terpinen-4-ol induces the highest disease resistance in strawberry compared with TTO and other components. Untargeted metabolomic analysis showed that terpinen-4-ol treatment strongly activated phenylpropanoid biosynthesis and flavonoid metabolism pathway by increasing the accumulation of cinnamaldehyde, coniferyl aldehyde, naringenin, taxifolin, quercetin, and quercitrin in fruit at 12 h after treatment. In addition, terpinen-4-ol treatment also caused the accumulation of total phenolics and lignin by enhancing activities and relative gene expression of key enzymes in the phenylpropanoid metabolism pathway. These results suggest that terpinen-4-ol, as the key component of TTO, is the most important contributor to the effectiveness of TTO in improving disease resistance of strawberry fruit through activating the phenylpropanoid metabolism pathway.