Novel Phr1 mutations and the evolution of phenol reaction variation in US weedy rice (Oryza sativa)
Novel Phr1 mutations and the evolution of phenol reaction variation in US weedy rice (Oryza sativa)
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DOI：
10.1111/j.1469-8137.2009.02957.x
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发表时间：
2009-01-01
期刊：
影响因子：
9.4
通讯作者：
Olsen, Kenneth M.
中科院分区：
文献类型：
--
作者：
Gross, Briana L.;Skare, Karl J.;Olsen, Kenneth M.
红米是一种主要的农业杂草，具有表型多样性，具有与野生稻和栽培稻相似的性状。可用于水稻的遗传资源使得有可能检查表征这种杂草的性状的分子基础和进化。在这里，我们在表型和分子水平上评估了红米中的苯酚反应（区分栽培稻品种的经典性状）。我们对100多个美国杂草样本的苯酚反应进行了表型分析，并对一部分样本中的潜在Phr 1基因座进行了测序。数据进行了分析，结合以前发表的Phr 1数据栽培rice.Most杂草加入（96.3%）是积极的苯酚反应，和样品的负响应携带的功能丧失的等位基因是罕见的或迄今为止没有记录。一个这样的等位基因可能已经通过1-bp移码插入的突变会聚而进化。红米和美国栽培品种之间的单倍型共享表明偶尔作物杂草杂交。我们发现以前没有记录的非功能性phr 1等位基因表明，可能有其他功能丧失突变分离在世界各地的水稻。红米可以提供一个有用的研究系统，了解Phr 1变异在农业环境中的适应意义。
P>Red rice, a major agricultural weed, is phenotypically diverse and possesses traits that are similar to both wild and cultivated rice. The genetic resources available for rice make it possible to examine the molecular basis and evolution of traits characterizing this weed. Here, we assess the phenol reaction - a classical trait for distinguishing among cultivated rice varieties - in red rice at the phenotypic and molecular levels.We phenotyped more than 100 US weed samples for the phenol reaction and sequenced the underlying Phr1 locus in a subset of samples. Data were analyzed in combination with previously published Phr1 data for cultivated rice.Most weed accessions (96.3%) are positive for the phenol reaction, and samples with a negative response carry loss-of-function alleles that are rare or heretofore undocumented. One such allele may have evolved through mutational convergence of a 1-bp frameshift insertion. Haplotype sharing between red rice and US cultivars suggests occasional crop-weed hybridization.Our discovery of previously undocumented nonfunctional phr1 alleles suggests that there are likely to be other loss-of-function mutations segregating in Oryza sativa around the world. Red rice may provide a useful study system for understanding the adaptive significance of Phr1 variation in agricultural settings.