METABOLISM STUDIES OF AN AZO DYE AND PIGMENT IN THE HAMSTER BASED ON ANALYSIS OF THE URINE FOR POTENTIALLY CARCINOGENIC AROMATIC AMINE METABOLITES
METABOLISM STUDIES OF AN AZO DYE AND PIGMENT IN THE HAMSTER BASED ON ANALYSIS OF THE URINE FOR POTENTIALLY CARCINOGENIC AROMATIC AMINE METABOLITES
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DOI：
10.1093/jat/4.3.132
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发表时间：
1980-01-01
影响因子：
2.5
通讯作者：
TOLOS, WP
中科院分区：
文献类型：
--
作者：
NONY, CR;BOWMAN, MC;TOLOS, WP
A single dose (100 mg/kg) of purified Direct Black 38, a benzidine-based azo dye, or Pigment Yellow 12, a 3,3''-dichlorobenzidine-based azo compound, was administered to hamsters to determine whether these chemicals are cleaved to potentially carcinogenic aromatic amines. These metabolism studies were conducted in accordance with an interagency agreement between the National Institute for Occupational Safety and Health and the Food and Drug Administration to assess the hazards that may occur if workers in the dye and pigment industries are occupationally exposed to these chemicals. Sensitive and specific assays of the urine by parallel electron-capture gas chromatography and high pressure liquid chromatography revealed the presence of high levels of benzidine, monoacetylbenzidine, diacetylbenzidine, 4-aminobiphenyl and alkaline hydrolyzable conjugates of benzidine and 4-aminobiphenyl during the first 8 h of excretion after administration of Direct Black 38. The levels of all metabolites and conjugates except monoacetylbenzidine and alkaline hydrolyzable conjugates of benzidine began to diminish markedly 16 h after administration of the dye and dissipated to background levels during the seven day test period. Appreciable residues of monoacetylbenzidine and alkaline hydrolyzable conjugates of benzidine were still present in the urine at the time that the experiment was terminated. Selected urine samples from treated animals were tested for mutagenic potential via the modified Ames Salmonella/microsome assay. Contrasting results were obtained from a limited study with Pigment Yellow 12. None of the anticipated metabolites were found in the urine at intervals up to 8 days after the pigment was administered. The results are in agreement with other similar laboratory studies. Although no evidence was found for in vivo cleavage of Pigment Yellow 12, the results with Direct Black 38, coupled with other data, indicate that workers exposed to this dye could be at risk of contracting bladder cancer.
