The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis
The small chemical vacuolin-1 alters the morphology of lysosomes without inhibiting Ca2+-regulated exocytosis
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DOI：
10.1038/sj.embor.7400495
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发表时间：
2005-09-01
期刊：
影响因子：
7.7
通讯作者：
Andrews, NW
中科院分区：
文献类型：
--
作者：
Huynh, C;Andrews, NW
钙离子调节的溶酶体胞吐作用是质膜创伤修复所必需的。小的化学物质液泡素-1改变了溶酶体的形态，而不影响细胞在损伤后重新密封质膜的能力。最近的一项研究发现，在经Vacuolin-1处理的细胞中，未能检测到钙离子触发的溶酶体胞吐作用，因此溶酶体对于再密封是不必要的。在这里，我们表明，尽管液泡素-1改变了溶酶体的形态，但并不抑制由于暴露于钙离子载体或质膜损伤而引起的溶酶体的胞吐。因此，在液泡素-1处理的细胞中，不能排除溶酶体作为膜修复的试剂。
Ca2+-regulated exocytosis of lysosomes was previously shown to be required for the repair of plasma membrane wounds. The small chemical vacuolin-1 alters the morphology of lysosomes without affecting the ability of cells to reseal their plasma membrane after injury. On the basis of a failure to detect Ca2+-triggered lysosomal exocytosis in vacuolin-1-treated cells, a recent study proposed that lysosomes are dispensable for resealing. Here, we show that vacuolin-1, despite altering lysosome morphology, does not inhibit the exocytosis of lysosomes induced by exposure to a Ca2+ ionophore, or by plasma membrane wounding. Thus, lysosomes cannot be excluded as agents of membrane repair in vacuolin-1-treated cells.