Both naturally occurring insertions of transposable elements and intermediate frequency polymorphisms at the achaete-scute complex are associated with variation in bristle number in Drosophila melanogaster.
Both naturally occurring insertions of transposable elements and intermediate frequency polymorphisms at the achaete-scute complex are associated with variation in bristle number in Drosophila melanogaster.
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发表时间：
2000-03
期刊：
影响因子：
3.3
通讯作者：
A. Long;R. F. Lyman;Alison H. Morgan;C. Langley;T. Mackay
中科院分区：
文献类型：
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作者：
A. Long;R. F. Lyman;Alison H. Morgan;C. Langley;T. Mackay
对包括无毛鳞片复合体（ASC）在内的110kb区域进行限制性内切酶调查，在56条自然存在的染色体样本中检测了14个多态性分子标记。大插入作为一个类别与胸胸膜和腹刚毛数量的减少有关，支持了维持定量遗传变异的有害突变选择平衡模型。两个多态位点独立地与在两个遗传背景下测量的刚毛数量的变化相关，通过排列测试进行评估。sc α附近6bp的缺失与两性胸膜刚毛数量的变化有关，sc β和sc γ之间3.4 kb的插入与女性腹部刚毛数量的变化有关。在加性遗传模型下，sc α附近的小缺失多态性占胸膜刚毛数量X染色体总遗传变异的25%，3.4 kb的插入占女性腹膜刚毛数量X染色体总变异的22%。与突变选择平衡模型相比，结合平衡选择模型或假设影响刚毛数量的变异是中性的模型更能简洁地解释与刚毛数量变化相关的常见多态性。
A restriction enzyme survey of a 110-kb region including the achaete scute complex (ASC) examined 14 polymorphic molecular markers in a sample of 56 naturally occurring chromosomes. Large insertions as a class were associated with a reduction in both sternopleural and abdominal bristle number, supporting deleterious mutation-selection equilibrium models for the maintenance of quantitative genetic variation. Two polymorphic sites were independently associated with variation in bristle number measured in two genetic backgrounds as assessed by a permutation test. A 6-bp deletion near sc alpha is associated with sternopleural bristle number variation in both sexes and a 3.4-kb insertion between sc beta and sc gamma is associated with abdominal bristle number variation in females. Under an additive genetic model, the small deletion polymorphism near sc alpha accounts for 25% of the total X chromosome genetic variation in sternopleural bristle number, and the 3.4 kb insertion accounts for 22% of the total X chromosome variation in female abdominal bristle number. The observation of common polymorphisms associated with variation in bristle number is more parsimoniously explained by models that incorporate balancing selection or assume variants affecting bristle number are neutral, than mutation-selection equilibrium models.