Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest Ethiopia
Prevalence and Antimicrobial Resistance in Salmonella and Shigella Species Isolated from Outpatients, Jimma University Specialized Hospital, Southwest Ethiopia
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DOI：
10.1155/2016/4210760
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发表时间：
2016-01-01
影响因子：
2.8
通讯作者：
Bacha, Ketema
中科院分区：
文献类型：
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作者：
Lamboro, Tesfahun;Ketema, Tsige;Bacha, Ketema
本研究旨在调查埃塞俄比亚西南部吉马大学专科医院门诊患者中沙门氏菌和志贺氏菌的流行情况。共有176名门诊患者参加了横断面研究。收集成人和儿童门诊患者的粪便样本，分析是否存在假定的沙门氏菌和志贺氏菌落，然后通过生化测试进行确认。沙门氏菌和志贺氏菌的纯培养进一步进行了对常用抗生素的耐药性测试。此外，按照标准程序对选定食品中的分离物的生长潜力进行了评估。结果表明，该地区门诊患者中沙门氏菌和志贺氏菌的感染率分别为19例(10.8%)和2例(1.1%)。此外，沙门氏菌对氨苄西林的耐药率为100%，其次为四环素(47.4%)和奈立地酸(26.3%)，志贺氏菌对氨苄西林和四环素的耐药率均为100%。在两种病原菌中都观察到对最多4种药物的多重耐药性。观察到病原体在24小时内生长到其感染量。总之，沙门氏菌和志贺氏菌仍然是研究地区对公共卫生具有重要意义的微生物之一。因此，除了提高广大公众对水卫生和卫生食品处理做法的认识外，还需要经常监测和评估其流行率和抗药性模式。
This study was designed to investigate the prevalence of Salmonella and Shigella among outpatients in Jimma University Specialized Hospital, Southwest Ethiopia. Cross-sectional study was conducted involving a total of 176 outpatients. Stool specimens from both adult and pediatric outpatients were collected and analyzed for the presence of presumptive Salmonella and Shigella colonies followed by confirmation by biochemical tests. Pure cultures of Salmonella and Shigella species were further subjected to test for antibiotic resistance against the commonly used antibiotics. Furthermore, growth potential of the isolates in selected foods items was assessed following standard procedures. The result indicated that the prevalence of Salmonella and Shigella among outpatients in the study area was 19 (10.8%) and 2 (1.1%), respectively. In addition, Salmonella species were resistant to ampicillin (100%) followed by tetracycline (47.4%) and nalidixic acid (26.3%) while Shigella species were highly resistant to ampicillin and tetracycline (100%, each). Multidrug resistance towards maximum of four drugs was observed in both pathogens. The pathogens were observed growing to their infective dose within 24 hours. In conclusion, Salmonella and Shigella are still among microbes of public health importance in the study area. Thus, this calls for frequent monitory and evaluation of their prevalence and drug resistance patterns besides awareness development on water sanitation and hygienic food handling practices to the public at large.