Mitotic arrest and toxicity in Biomphalaria glabrata (Mollusca: Pulmonata) exposed to colchicine.
Mitotic arrest and toxicity in Biomphalaria glabrata (Mollusca: Pulmonata) exposed to colchicine.
复制标题
暴露于秋水仙碱的光滑双脐藻（软体动物：肺）有丝分裂停滞和毒性。
DOI：
10.1016/j.jip.2005.04.005
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Castro,Lina
中科院分区：
文献类型：
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作者：
Sullivan,JohnT;Castro,Lina
与未暴露的蜗牛相比，连续暴露于 0.1% 秋水仙碱的光滑双脐螺在 4 小时内其变形细胞生成器官 (APO) 中的有丝分裂象数显着增加，在暴露 12 小时后达到峰值。 24小时时循环血细胞数量显着增加。然而，到 72 小时，APO 中的有丝分裂像数量和血淋巴中循环血细胞的浓度都恢复到控制水平。在整个暴露过程中，血细胞似乎具有正常的形态，包括形成长丝状伪足和支撑杆状结构，据报道，这些丝状伪足含有微管。蜗牛的存活率随着暴露时间的增加而降低。与对照组相比，在暴露于 0.1% 秋水仙碱 72 小时后，能够存活 48 小时的蜗牛数量明显减少，并且只有十分之一的蜗牛从暴露于 0.1% 秋水仙碱的 72 小时中恢复过来。暴露于秋水仙碱的蜗牛即使从秋水仙碱溶液中取出后也表现出中毒行为，尽管暴露 72 小时的蜗牛的中枢神经系统没有明显的组织病理学变化。
Continuous exposure of Biomphalaria glabrata snails to 0.1% colchicine resulted in a significant increase, relative to non-exposed snails, in the number of arrested mitotic figures in the amebocyte-producing organ (APO) as soon as 4h, with peak numbers after 12h of exposure. The number of circulating hemocytes was significantly elevated at 24h. However, by 72h both the number of mitotic figures in the APO and the concentration of circulating hemocytes in the hemolymph had returned to control levels. Hemocytes appeared to possess normal morphology throughout this exposure, including the formation of long filopodia with supporting rodlike structures that have been reported to contain microtubules. Snail survival decreased as a function of exposure time. Significantly fewer snails, relative to controls, survived a 48-h exposure, and only 1 out of 30 snails recovered from a 72-h exposure to 0.1% colchicine. Colchicine-exposed snails displayed intoxicated behavior, even upon removal from the colchicine solution, although no histopathology was evident in the CNS of snails exposed for 72h.