Cell culture-produced hepatitis C virus does not infect peripheral blood mononuclear cells.
Cell culture-produced hepatitis C virus does not infect peripheral blood mononuclear cells.
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DOI：
10.1002/hep.22550
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发表时间：
2008-12
期刊：
影响因子：
13.5
通讯作者：
Dustin, Lynn B.
中科院分区：
文献类型：
--
作者：
Marukian, Svedana;Jones, Christopher T.;Andrus, Linda;Evans, Matthew J.;Ritola, Kimberly D.;Charles, Edgar D.;Rice, Charles M.;Dustin, Lynn B.
丙型肝炎病毒（HCV）主要在肝脏中复制，但已观察到HCV RNA与其他组织和细胞（包括B和T淋巴细胞、单核细胞和树突状细胞）相关。我们已经利用最近描述的，强大的系统，完全重演HCV进入，复制和病毒生产在体外重新检查血细胞亚群的HCV感染的问题。HCV复制酶抑制剂2 'C-甲基腺苷用于区分HCV RNA复制和RNA持久性。虽然细胞培养生长的HCV在Huh-7.5肝癌细胞中复制，但在健康供体的B或T淋巴细胞、单核细胞、巨噬细胞或树突状细胞中未检测到HCV复制。没有检测到血细胞亚群表达显著水平的Claudin-1，一种HCV感染Huh-7.5细胞所需的紧密连接蛋白。表达高水平紧密连接蛋白-1、CD 81和SR-BI的B细胞系对HCV假颗粒感染保持抗性。我们通过将HCV RNA导入血细胞亚群来绕过HCV进入的障碍。转染的RNA没有可检测的翻译，并诱导高水平的IFNα。HCVRNA转染的巨噬细胞的上清液抑制Huh-7.5细胞中的HCV复制。我们的结论是，多块阻止血细胞支持HCV感染。
Hepatitis C virus (HCV) replicates primarily in the liver, but HCV RNA has been observed in association with other tissues and cells including B and T lymphocytes, monocytes, and dendritic cells. We have taken advantage of a recently described, robust system that fully recapitulates HCV entry, replication and virus production in vitro to re-examine the issue of HCV infection of blood cell subsets. The HCV replicase inhibitor 2’C-methyladenosine was used to distinguish HCV RNA replication from RNA persistence. While cell culture-grown HCV replicated in Huh-7.5 hepatoma cells, no HCV replication was detected in B or T lymphocytes, monocytes, macrophages, or dendritic cells from healthy donors. No blood cell subset tested expressed significant levels of Claudin-1, a tight junction protein needed for HCV infection of Huh-7.5 cells. A B cell line expressing high levels of Claudin-1, CD81, and SR-BI remained resistant to HCV pseudoparticle infection. We bypassed the block in HCV entry by transfecting HCV RNA into blood cell subsets. Transfected RNA was not detectably translated and induced high levels of IFNα. Supernatants from HCV RNA-transfected macrophages inhibited HCV replication in Huh-7.5 cells. We conclude that multiple blocks prevent blood cells from supporting HCV infection.
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DOI：
10.1073/pnas.0504877103
发表时间：
2006-05-09
影响因子：
11.1
作者：
Pietschmann, Thomas;Kaul, Artur;Bartenschlager, Ralf
通讯作者：
Bartenschlager, Ralf
影响因子：
12.7
作者：
Fournillier, A;Freida, D;Inchauspé, G
通讯作者：
Inchauspé, G
影响因子：
5.4
作者：
Grove, Joe;Huby, Thierry;McKeating, Jane A.
通讯作者：
McKeating, Jane A.
影响因子：
5.4
作者：
Meertens, Laurent;Bertaux, Claire;Dragic, Tatjana
通讯作者：
Dragic, Tatjana
影响因子：
5.4
作者：
Catanese, Maria Teresa;Graziani, Rita;Nicosia, Alfredo
通讯作者：
Nicosia, Alfredo