Phage-Based Structural Color Sensors and Their Pattern Recognition Sensing System
Phage-Based Structural Color Sensors and Their Pattern Recognition Sensing System
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DOI：
10.1021/acsnano.6b07942
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发表时间：
2017-04-01
期刊：
影响因子：
17.1
通讯作者：
Lee, Seung-Wuk
中科院分区：
文献类型：
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作者：
Lee, Ju Hun;Fan, Benson;Lee, Seung-Wuk
哺乳动物的嗅觉系统为智能传感器的设计提供了巨大的灵感。为此，我们开发了基于生物灵感的噬菌体纳米结构颜色传感器阵列和基于智能手机的传感网络系统。使用M13噬菌体(噬菌体)作为基本构建块，我们创建了由自组装噬菌体的液晶束纳米纤维组成的结构颜色矩阵。这些噬菌体被设计成在其主要外壳蛋白(PVIII)上表达交叉反应受体，导致接触各种目标化学物质时快速、可检测的颜色变化，从而产生依赖于化学和浓度的颜色指纹。使用这些传感器，我们成功地检测到了5-90%的相对湿度，灵敏度为0.2%。此外，经过芳香族受体修饰后，我们能够区分各种结构相似的有毒化学物质，包括苯、甲苯、二甲苯和苯胺。此外，我们还开发了一种使用智能手机来解释和传播这些传感器的结果的方法，以建立一个无线系统。我们的基于噬菌体的传感器系统在改善国家安全、监测环境和人类健康方面具有非常有用的潜力。
The mammalian olfactory system provides great inspiration for the design of intelligent sensors. To this end, we have developed a bioinspired phage nanostructure-based color sensor array and a smartphonebased sensing network system. Using a M13 bacteriophage (phage) as a basic building block, we created structural color matrices that are composed of liquid-crystalline bundled nanofibers from self-assembled phages. The phages were engineered to express cross-responsive receptors on their major coat protein (pVIII), leading to rapid, detectable color changes upon exposure to various target chemicals, resulting in chemical- and concentration-dependent color fingerprints. Using these sensors, we have successfully detected 5-90% relative humidity with 0.2% sensitivity. In addition, after modification with aromatic receptors, we were able to distinguish between various structurally similar toxic chemicals including benzene, toluene, xylene, and aniline. Furthermore, we have developed a method of interpreting and disseminating results from these sensors using smartphones to establish a wireless system. Our phage-based sensor system has the potential to be very useful in improving national security and monitoring the environment and human health.