Dual-signal output paper sensor based on coordinative self-assembly biomimetic nanozyme for point-of-care detection of biomarker
Dual-signal output paper sensor based on coordinative self-assembly biomimetic nanozyme for point-of-care detection of biomarker
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DOI：
10.1016/j.bios.2022.114656
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发表时间：
2022-09-09
影响因子：
12.6
通讯作者：
Feng, Guangfu
中科院分区：
文献类型：
--
作者：
Du, Yuanchun;Ke, Zhenyi;Feng, Guangfu
基于纸的床旁检测（POC）设备具有简单、快速、尺寸小和成本低的优点，这对于食品安全、生物分析和医学诊断特别重要。然而，用于制造纸基POC的材料很少产生多个信号，阻碍了在不同情况下的进一步应用。在此，我们提出了一种新的方法，即比色-温度双信号输出传感器（CTDSS），并构建了一种基于协同自组装仿生纳米酶FeGMP-L-His CPNs的CTDSS作为概念验证。这些CPNs模拟辣根过氧化物酶（HRP）的结构，其中Fe（II）是中心，核苷酸GMP和组氨酸被选择为配体以分别模拟HRP中吡咯环的金属配位和蛋白质功能。这种策略使CPNs表现出优异的过氧化物酶样活性，有效地将H2 O2转化为中心点OH并氧化TMB产生比色-温度双信号。作为一个概念验证应用，我们利用胆固醇作为目标，并成功地应用这种CTDSS检测胆固醇，显示出非凡的快速性，显着的特异性和高灵敏度的特点。利用比色测试条和温度变色贴，构建了基于纸的POC工具来可视化目标。同时，两种产生不同信号输出的测试条POC装置显示出显著的可行性，并进一步用于检测人血清中的胆固醇。我们预计，这个CTDSS平台将激发未来便携式检测工具的创新概念。
Paper-based point-of-care (POC) devices exhibit the advantages of simplicity, rapidity, trim sizes, and low cost, which are of particular importance for food safety, biological analysis, and medical diagnosis. However, the materials utilized to make paper-based POC rarely produce multiple signals, hampering further applications in diverse situations. Herein, we present an appealing approach, namely Colorimetric-Temperature Dual-Signal Output Sensor (CTDSS), and construct a CTDSS based on coordinative self-assembly biomimetic nanozymes FeGMP-L-His CPNs as a proof of concept. These CPNs mimic the structure of horseradish peroxidase (HRP), in which Fe (II) is the center, nucleotide GMP and histidine are chosen as ligands to simulate metal coordination of the pyrrole ring and protein function in HRP, respectively. This strategy allows CPNs to show an excellent peroxidase-like activity, efficiently converting H2O2 into center dot OH and oxidizing TMB to generate colorimetrictemperature dual-signal. As a proof-of-concept application, we exploited cholesterol as the target and successfully applied this CTDSS to detect cholesterol, displaying extraordinary features of rapidity, dramatic specificity, and high sensitivity. By utilizing the colorimetric test strip and temperature discoloration sticker, the paperbased POC tools were constructed to visualize the target. Meanwhile, two proposed test strip POC devices generating different signal outputs exhibited remarkable feasibility and were further employed to detect cholesterol in human serum. We anticipate that this CTDSS platform will inspire innovative concepts for future portable detection tools.