Resting-state functional connectivity analysis of the mouse brain using intrinsic optical signal imaging of cerebral blood volume dynamics
Resting-state functional connectivity analysis of the mouse brain using intrinsic optical signal imaging of cerebral blood volume dynamics
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DOI：
10.1088/1361-6579/aac033
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发表时间：
2018-05
影响因子：
3.2
通讯作者：
Yuto Yoshida;M. Nakao;N. Katayama
中科院分区：
文献类型：
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作者：
Yuto Yoshida;M. Nakao;N. Katayama
目的：人脑静息态功能连接（rsFC）与神经精神疾病密切相关。由于侵入性实验技术的适用性，小鼠被广泛用于研究此类疾病的生理机制。因此，对小鼠大脑rsFC的研究对于将生理机制与人类的这些疾病联系起来至关重要。在这项研究中，我们调查的适用性的内在光学信号成像的脑血容量（IOSI-CBV）的rsFC分析小鼠大脑。方法：使用冷却CCD相机从未麻醉的野生型小鼠的脑中收集经颅IOSI-CBV图像。所有像素的时间轨迹被平均以创建全局信号（GS）。进行边缘和偏相关分析以基于具有和不具有GS去除的CBV信号来估计rsFC。通过将结果与先前研究中报告的rsFC数据进行比较，证实了结果的一致性。主要结果：我们证实GS与心率波动在FC频段。CBV与GS去除的边缘相关系数与使用依赖于氧合血红蛋白浓度和脑血流量的传统光学成像方法的测量结果一致。意义：这些结果表明经颅IOSI-CBV方法估计小鼠脑的rsFC的适用性和有用性。
Objective: Resting-state functional connectivity (rsFC) of the human brain is closely related with neurological and psychiatric disorders. Mice are widely used to investigate the physiological mechanisms of such disorders, because of the applicability of invasive experimental techniques. Thus, studies on rsFC of the mouse brain are essential to link physiological mechanisms with these disorders in humans. In this study, we investigated the applicability of intrinsic optical signal imaging of cerebral blood volume (IOSI-CBV) for rsFC analysis of the mouse brain. Approach: Transcranial IOSI-CBV images were collected from the brains of un-anesthetized wild-type mice with a cooled-CCD camera. The time traces of all pixels were averaged to create a global signal (GS). Marginal and partial correlation analyses were performed to estimate the rsFC based on CBV signals both with and without GS removal. The consistency of the results were confirmed by comparing them with to the rsFCs data reported in the previous studies. Main results: We confirmed that GS correlated with heart rate fluctuation in the FC frequency band. The marginal correlation coefficient of CBV with GS removal was consistent with measurements using conventional optical imaging methods relying on oxygenated hemoglobin concentration and cerebral blood flow. Significance: These results suggest the applicability and usefulness of the transcranial IOSI-CBV method to estimate rsFC of the mouse brain.