Engraftment of HTLV-I-transformed human T-cell line into SCID mice with NK cell function.
Engraftment of HTLV-I-transformed human T-cell line into SCID mice with NK cell function.
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将 HTLV-I 转化的人类 T 细胞系移植到具有 NK 细胞功能的 SCID 小鼠中。
DOI：
10.1292/jvms.56.601
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
T. Nomura
中科院分区：
文献类型：
--
作者：
T. Ohsugi;K. Ishibashi;M. Shingu;T. Nomura
已报道HTLV-I转化的人T细胞系MT-2仅在具有NK细胞功能耗竭的SCID小鼠中成功增殖。然而，MT-2细胞在本研究中可以移植到具有正常NK细胞功能的SCID小鼠中。将MT-2细胞（5-7 × 10（7）细胞）腹膜内注射到SCID小鼠中，而不缺失NK细胞功能。在接种MT-2细胞后3个月，SCID小鼠在腹膜腔中产生肿瘤。所有肿瘤均对抗HTLV-Ⅰ p19和抗HLA-DR单克隆抗体有免疫荧光反应，PCR检测HTLV-Ⅰ基因也呈阳性。从肿瘤组和非肿瘤组小鼠的主要器官中获得的DNA经PCR检测显示HTLV-1基因的阳性信号的发生率高。
HTLV-I-transformed human T-cell line, MT-2, has been reported to successfully proliferate in only the SCID mice with depletion of NK cell function. However, MT-2 cells could be engrafted into SCID mice possessing normal NK cell function in this study. MT-2 cells (5-7 x 10(7) cells) were intraperitoneally injected into SCID mice without the deletion of NK cell function. The SCID mice developed tumors in the peritoneal cavities 3 months after the inoculation of MT-2 cells. All tumors reacted to anti-HTLV-I p19 and anti-HLA-DR monoclonal antibodies by immunofluorescence assay and were also positive for the HTLV-I gene by PCR assay. DNA obtained from main organs in group of mice with or without tumors showed a high incidence of positive signals for HTLV-I gene by PCR assay.