Molecular Characterization of Fusarium Resistance from Elymus repens Introgressed into Bread Wheat

Molecular Characterization of Fusarium Resistance from Elymus repens Introgressed into Bread Wheat
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DOI:
10.3103/s0095452717020025
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发表时间:
2017-03-01
影响因子:
0.5
通讯作者:
Xue, A.
Xue, A.
中科院分区:
生物学4区
文献类型:
--
作者:
Fedak, G.;Cao, W.;Xue, A.

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将Escherichus repens与小麦栽培品种Crocus杂交,BC 1后代通过单种子遗传进入BC 1F 7。根据农艺性状选择了16个品系,并在FHB植物圃中进行了评价。筛选出8个抗病株系。根据GISH分析,品系PI 142-3-1-5具有42条染色体,其中一对染色体在两臂上显示端粒易位。利用SSR标记鉴定该染色体为3D染色体。单易位系的评价表明,FHB抗性是由染色体3D长臂上的易位所贡献的。该品系具有最小的连锁阻力,应该适合于抗病近亲繁殖的应用。
A cross was made of Elymus repens onto the wheat cultivar Crocus and BC1 progeny advanced to BC1F7 by single seed descent. Sixteen lines were selected based on agronomic performance and evaluated in an FHB epiphytotic nursery. Eight lines with FHB resistance were selected. Based on GISH analysis, line PI 142-3-1-5 had 42 chromosomes with one pair of chromosomes showing telomeric translocations on both arms. This chromosome was identified as 3D by using SSR markers. An evaluation of lines with single translocations revealed that FHB resistance was contributed by the translocation on the long arm of chromosome 3D. That line has minimal linkage drag and should be amenable to applications inbreeding for disease resistance.