Leifsonia rubra sp. nov. and Leifsonia aurea sp. nov., psychrophiles from a pond in Antarctica.
Leifsonia rubra sp. nov. and Leifsonia aurea sp. nov., psychrophiles from a pond in Antarctica.
复制标题
DOI：
10.1099/ijs.0.02396-0
复制
发表时间：
2003-07
影响因子：
2.8
通讯作者：
G. Reddy;J. Prakash;R. Srinivas;G. Matsumoto;S. Shivaji
中科院分区：
文献类型：
--
作者：
G. Reddy;J. Prakash;R. Srinivas;G. Matsumoto;S. Shivaji
从南极洲麦克默多赖特谷的一个池塘中分离到两个独特的嗜冷菌株（CMS 76 rT和CMS 81 yT）。这两个分离物被指定为Leifsonia属，因为它们是革兰氏阳性、弯曲杆、非运动、过氧化氢酶阳性，含有DL-2，4-二氨基丁酸、甲基萘醌MK-11、磷脂酰甘油和二磷脂酰甘油，具有高含量的反异构和异构化脂肪酸，并且具有64-66摩尔%的DNA G + C含量。此外，这两个分离物都与5个已报道的Leifsonia属物种在约95-96%的16 S rDNA序列相似性水平上相关，并且彼此相差2.5%。CMS 76 rT和CMS 81 yT在许多其他表型特征上也彼此不同，并且在DNA-DNA水平上仅显示出30%的相关性，因此表明它们代表两个不同的物种。此外，这两个分离物在表型特征、生化特性、化学分类学特征、对各种抗生素的敏感性和16 SrDNA相似性方面与已报道的Leifsonia种也显示出许多明显的差异，清楚地表明菌株CMS 76 rT（= MTCC 4210 T = DSM 15304 T = CIP 107783 T）和CMS 81 yT（= MTCC 4657 T = DSM 15303 T = CIP 107785 T）代表了两个新的Leifsonia种的模式菌株，并建议将其命名为Leifsonia rubra sp. nov.和Leifsonia aurea sp. nov.。
Two unique psychrophilic strains (CMS 76rT and CMS 81yT) were isolated from a cyanobacterial mat sample from a pond in Wright Valley, McMurdo, Antarctica. Both isolates were assigned to the genus Leifsonia, since they were gram-positive, curved rods, non-motile, catalase-positive, contained DL-2,4-diaminobutyric acid, menaquinone MK-11, phosphatidylglycerol and diphosphatidylglycerol, had a high content of anteiso- and iso-branched fatty acids and had a DNA G + C content of 64-66 mol%. In addition, both isolates were related to the five reported species of Leifsonia at a level of about 95-96% 16S rDNA sequence similarity and differed from one another by 2.5%. Strains CMS 76rT and CMS 81yT also differed from one another in many other phenotypic characteristics and exhibited only 30% relatedness at the DNA-DNA level, thus indicating that they represent two different species. Furthermore, these two isolates also showed many distinct differences with respect to the reported species of Leifsonia in terms of their phenotypic characteristics, biochemical properties, chemotaxonomic features, sensitivity to various antibiotics and 16S rDNA similarity, clearly indicating that strains CMS 76rT (= MTCC 4210T = DSM 15304T = CIP 107783T) and CMS 81yT (= MTCC 4657T = DSM 15303T = CIP 107785T) represent the type strains of two novel species of Leifsonia, for which the names Leifsonia rubra sp. nov. and Leifsonia aurea sp. nov. are proposed.