MEIOTIC MATURATION IN CULTURED BOVINE OOCYTES IS ACCOMPANIED BY REMODELING OF THE CUMULUS CELL CYTOSKELETON
MEIOTIC MATURATION IN CULTURED BOVINE OOCYTES IS ACCOMPANIED BY REMODELING OF THE CUMULUS CELL CYTOSKELETON
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DOI：
10.1006/dbio.1993.1171
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发表时间：
1993-07-01
影响因子：
2.7
通讯作者：
ALBERTINI, DF
中科院分区：
文献类型：
--
作者：
ALLWORTH, AE;ALBERTINI, DF
哺乳动物卵母细胞成熟和卵丘扩张是激素诱导的同时过程，涉及卵丘细胞形状的改变和卵丘细胞与卵母细胞之间的相互作用。用激光扫描共聚焦显微镜和常规荧光显微镜研究了牛卵丘卵母细胞复合体(COCs)体外成熟过程中卵丘细胞骨架重组与减数分裂进程的关系。在成熟培养液中加入激素补充剂(FSH、LH、E_2)是减数分裂进行到中期2和最大卵丘扩展所必需的。在这些条件下，观察到了卵丘细胞跨带突起的细胞骨架含量和组织的阶段特异性变化。具体地说，在GV期COC中观察到丰富的、充满肌动蛋白的跨带突起。在成熟过程的中间阶段(培养10小时)，较少看到充满肌动蛋白的跨带突起，而微管充满的跨带突起变得明显。在成熟末期(24小时培养)，大量细长的微管丰富的跨带突起明显，很少观察到充满肌动蛋白的突起。此外，在所研究的所有阶段的卵母细胞中，都一致地观察到富含微管的跨带突起与卵母细胞染色质之间的空间关系。这些结果表明，牛卵母细胞在培养中的成熟涉及卵丘细胞细胞骨架的改变，这可能调节卵母细胞成熟的开始、进展和完成。
Mammalian oocyte maturation and cumulus expansion are hormone-induced contemporaneous processes that involve changes in cumulus cell shape and in the interactions between cumulus cells and the oocyte. Laser scanning confocal and conventional fluorescence microscopy have been used to study the relationship between reorganization of the cumulus cell cytoskeleton and meiotic progression in bovine cumulus oocyte complexes (COCs) maturedin vitro. Inclusion of hormone supplements (FSH, LH, E2) in the maturation media was required for meiotic progression to metaphase 2 and maximal cumulus expansion. Under these conditions, stage-specific changes in the cytoskeletal content and organization of cumulus cell transzonal processes was observed. Specifically, abundant, actin-filled transzonal processes were observed in GV stage COCs. In stages intermediate in the maturation process (10 hr culture), fewer actin-filled transzonal processes were seen and microtubule-filled transzonal processes became apparent. At the end of maturation (24 hr culture), numerous, elongate microtubule rich transzonal processes were evident and actin-filled processes were rarely observed. Additionally, a spatial relationship between microtubule rich transzonal processes and oocyte chromatin was consistently observed in all stages of oocytes examined. These results indicate that bovine oocyte maturation in culture involves modifications of the cumulus cell cytoskeleton that may regulate the onset, progression, and completion of oocyte maturation.