Fungal and bacterial utilization of organic substrates depends on substrate complexity and N availability
Fungal and bacterial utilization of organic substrates depends on substrate complexity and N availability
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DOI：
10.1111/1574-6941.12214
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发表时间：
2014-01-01
影响因子：
4.2
通讯作者：
Richter, Andreas
中科院分区：
文献类型：
--
作者：
Koranda, Marianne;Kaiser, Christina;Richter, Andreas
越来越多的证据表明，微生物群落的组成和功能之间存在直接关系，这意味着不同的微生物群落在其功能特性方面存在差异。本研究的目的是确定不同的微生物群落之间的初始底物利用率的差异是否是由于某些微生物群的活动。我们进行了一个短期的实验，山毛榉森林土壤的特点是由三个不同的微生物群落（冬季和夏季社区，和社区从树木环剥情节）。我们孵育这些土壤与不同的C-13标记的基板有或无无机氮添加和分析微生物底物利用C-13-磷脂脂肪酸（PLFA）分析。我们的研究结果表明，不稳定的C（葡萄糖）的命运是相似的，在三个微生物群落，尽管夏季和冬季社区之间的绝对底物掺入的差异。参与降解复杂的C基质（纤维素，植物细胞壁）的活性微生物群落，然而，不同的环剥和控制地块，并强烈影响无机氮添加。提高氮的有效性强烈增加纤维素和植物细胞壁的真菌降解。我们的研究结果表明，真菌，至少在高氮供应的存在下，是聚合物C基板的主要分解者。
There is growing evidence of a direct relationship between microbial community composition and function, which implies that distinct microbial communities vary in their functional properties. The aim of this study was to determine whether differences in initial substrate utilization between distinct microbial communities are due to the activities of certain microbial groups. We performed a short-term experiment with beech forest soils characterized by three different microbial communities (winter and summer community, and a community from a tree-girdling plot). We incubated these soils with different C-13-labelled substrates with or without inorganic N addition and analyzed microbial substrate utilization by C-13-phospholipid fatty acid (PLFA) analysis. Our results revealed that the fate of labile C (glucose) was similar in the three microbial communities, despite differences in absolute substrate incorporation between the summer and winter community. The active microbial community involved in degradation of complex C substrates (cellulose, plant cell walls), however, differed between girdling and control plots and was strongly affected by inorganic N addition. Enhanced N availability strongly increased fungal degradation of cellulose and plant cell walls. Our results indicate that fungi, at least in the presence of a high N supply, are the main decomposers of polymeric C substrates.