Depth-resolved fiber photometry with a single tapered optical fiber implant
Depth-resolved fiber photometry with a single tapered optical fiber implant
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DOI：
10.1038/s41592-019-0581-x
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发表时间：
2019-11-01
期刊：
影响因子：
48
通讯作者：
Pisanello, Ferruccio
中科院分区：
文献类型：
--
作者：
Pisano, Filippo;Pisanello, Marco;Pisanello, Ferruccio
纤维光度学越来越多地被用于监测大脑中神经活动的荧光传感器。然而，大多数实施方式是基于只能与光纤附近的浅组织体积对接的平裂光纤。我们利用锥形光纤(TF)的模式特性来实现组织范围达2毫米的光收集和沿锥形的多点光度测量。使用单一的转运蛋白，我们同时观察到在执行简单的可操作性条件反射任务的自由活动小鼠的背侧和腹侧纹状体中不同的多巴胺瞬变。还可以通过对锥形的非平面表面进行微结构来设计来自TF的收集体积的形状和大小，从而不仅在大脑深处而且通常在任何生物系统或器官中光学定位于多个位置，在这些系统或器官中，由于光的散射和吸收，光收集是有益的但具有挑战性。
Fiber photometry is increasingly utilized to monitor fluorescent sensors of neural activity in the brain. However, most implementations are based on flat-cleaved optical fibers that can only interface with shallow tissue volumes adjacent to the fiber. We exploit modal properties of tapered optical fibers (TFs) to enable light collection over an extent of up to 2 mm of tissue and multisite photometry along the taper. Using a single TF, we simultaneously observed distinct dopamine transients in dorsal and ventral striatum in freely moving mice performing a simple, operant conditioning task. Collection volumes from TFs can also be engineered in both shape and size by microstructuring the nonplanar surface of the taper, to optically target multiple sites not only in the deep brain but, in general, in any biological system or organ in which light collection is beneficial but challenging because of light scattering and absorption.