Repair of Osteochondral Defects Using Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells in a Rabbit Model.
Repair of Osteochondral Defects Using Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells in a Rabbit Model.
复制标题
DOI：
10.1155/2017/8760383
复制
发表时间：
2017
影响因子：
--
通讯作者：
Guo Q
中科院分区：
文献类型：
--
作者：
Liu S;Jia Y;Yuan M;Guo W;Huang J;Zhao B;Peng J;Xu W;Lu S;Guo Q
脐带Wharton的凝胶源间充质干细胞(WJMSC)是近年来发现的具有多向分化潜能的间充质干细胞。与其他成人MSCs相比，WJMSC来源丰富，无损伤获取，免疫原性低，有望成为组织工程中良好的异种细胞候选者。这项体内初步研究探索了利用含有组织工程构建物的人脐Wharton‘s凝胶间充质干细胞(HWJMSCs)异种移植修复股骨髌骨沟全层软骨缺损的方法。在超过16个月的修复过程中，我们观察到hWJMSCs有序的时空重塑为软骨组织，具有特征性的建筑特征，包括表面规则的透明样新骨层，与邻近宿主软骨完全结合，以及再生的软骨下骨。将hWJMSCs移植到兔软骨缺损区，未检测到免疫排斥反应。通过大体观察和组织学分级评分，hWJMSCs的修复效果优于软骨诱导的hWJMSCs。这些初步结果提示，使用新型未分化的hWJMSCs作为种子细胞可能比使用转化生长因子-β诱导的分化的hWJMSCs更适合体内组织工程治疗软骨缺损。HWJMSC同种异体移植有望在临床应用。
Umbilical cord Wharton's jelly-derived mesenchymal stem cell (WJMSC) is a new-found mesenchymal stem cell in recent years with multiple lineage potential. Due to its abundant resources, no damage procurement, and lower immunogenicity than other adult MSCs, WJMSC promises to be a good xenogenous cell candidate for tissue engineering. This in vivo pilot study explored the use of human umbilical cord Wharton's jelly mesenchymal stem cells (hWJMSCs) containing a tissue engineering construct xenotransplant in rabbits to repair full-thickness cartilage defects in the femoral patellar groove. We observed orderly spatial-temporal remodeling of hWJMSCs into cartilage tissues during repair over 16 months, with characteristic architectural features, including a hyaline-like neocartilage layer with good surface regularity, complete integration with adjacent host cartilage, and regenerated subchondral bone. No immune rejection was detected when xenograft hWJMSCs were implanted into rabbit cartilage defects. The repair results using hWJMSCs were superior to those of chondrogenically induced hWJMSCs after assessing gross appearance and histological grading scores. These preliminary results suggest that using novel undifferentiated hWJMSCs as seed cells might be a better approach than using transforming growth factor-β-induced differentiated hWJMSCs for in vivo tissue engineering treatment of cartilage defects. hWJMSC allografts may be promising for clinical applications.