Variability of exhaled breath condensate leukotriene B4 and 8-isoprostane in COPD patients.
Variability of exhaled breath condensate leukotriene B4 and 8-isoprostane in COPD patients.
复制标题
DOI：
10.2147/copd.2007.2.1.71
复制
发表时间：
2007
影响因子：
2.8
通讯作者：
Singh SD
中科院分区：
文献类型：
--
作者：
Borrill ZL;Starkey RC;Singh SD
呼出气冷凝液（EBC）介质的重现性在慢性阻塞性肺疾病（COPD）中没有很好的记录。本研究评估了EBC白三烯B4（LTB 4）和8-异前列烷的测定内（WA）、测定内（WD）和测定日间（BD）重现性。从24例COPD患者中采集3份EBC样本，间隔1 h和1 wk，以评估WD和BD的重现性。通过酶免疫分析一式三份的样品分析评估WA再现性。LTB 4和8-异前列烷的WA变异系数（分别为18.2%和29.2%）低于WD（分别为47.7%和65.3%）和BD（分别为75.7%和79.1%）的相应值。8-异前列烷和LTB 4测定的重复性系数分别为18.6 pg/ml和13.2 pg/ml。WD和BD的组平均差异较小且无统计学显著性。使用Bland Altman方法，WD（8-异前列烷为−51.6至47.2，LTB 4为−31.8至31.4）和BD重现性（8-异前列烷为−61.4至75.7，LTB 4为−29.3至38.6）的一致性范围较宽。这是第一项全面报告EBC 8-异前列烷和LTB 4在COPD中的变异性的研究。WA变异性和组平均变化较小。然而，我们观察到相当大的WD和BD的变异性，这些生物标志物。
The reproducibility of exhaled breath condensate (EBC) mediators is not well documented in chronic obstructive pulmonary disease (COPD). This study assessed within assay (WA), within (WD) and between day (BD) reproducibility of EBC leukotriene B4 (LTB4) and 8-isoprostane. Three EBC samples were collected from 24 COPD patients separated by 1 h and 1 wk, to assess WD and BD reproducibility. WA reproducibility was assessed by sample analysis by enzyme immunoassay in triplicate. WA coefficient of variation for LTB4 and 8-isoprostane (18.2% and 29.2%, respectively) was lower than corresponding values for WD (47.7% and 65.3%, respectively) and BD (75.7% and 79.1%, respectively). Repeatability coefficient for 8-isoprostane and LTB4 assays were 18.6 pg/ml and 13.2 pg/ml, respectively. Group mean differences for WD and BD were small and statistically nonsignificant. Using the Bland Altman method, there were wide limits of agreement for WD (−51.6 to 47.2 for 8-isoprostane and −31.8 to 31.4 for LTB4) and BD reproducibility (−61.4 to 75.7 for 8-isoprostane and −29.3 to 38.6 for LTB4). This is the first study to fully report the variability of EBC 8-isoprostane and LTB4 in COPD. WA variability and group mean changes were small. However, we observed considerable WD and BD variability for these biomarkers.